Adrenocortical cancer (ACC) is a rare aggressive malignancy. Recent ACC integrated genomics analysis contributed to redefine the risk groups on molecular basis, including tumor microRNAs (miRs), detectable also in the bloodstream. We developed a quantitative real-time (RT) assay for the measurement of miR483 and miR483-5p absolute levels in plasma samples. miR483/miR483-5p levels were evaluated in plasma samples of 27 patients with ACC before surgery and at follow-up. Statistically significant differences in miR483-5p and miR483 levels were found between stage 1/2 and stage 3/4 ACCs in pre-surgery and post-surgery samples. ROC curve analysis of miR483–5p levels gave a prediction of the clinical stage (accuracy 0.917±0.084), with the best cut-off value of 0.221 ng/ml, prognosticating overall and recurrence-free survival. In a multivariate Cox analysis (HR 16.2, 95%CI[1.39-188.6, P<0.026]). miR483-5p was the only variable that significantly predicted recurrence, but not overall survival, In addition, miR483 and miR483-5p levels correlated with the number of circulating tumor cells (CTCs) detected in the same blood samples, independently of the timing of sampling. In conclusion, we demonstrated that miR483-5p absolute plasma levels in ACC patients are powerful molecular markers that may help in the follow-up of patients after surgery and chemotherapy, and contribute to more accurately classify and predict tumor progression.

NEW INSIGHTS IN THE CLINICAL AND TRANSLATIONAL RELEVANCE OF miR483-5p IN ADRENOCORTICAL CANCER / Salvianti, Francesca; Canu, Letizia; Poli, Giada; Armignacco, Roberta; Scatena, Cristian; Cantini, Giulia; Franco, Alessandra Di; Gelmini, Stefania; Ercolino, Tonino; Pazzagli, Mario; Nesi, Gabriella; Mannelli, Massimo; Pinzani, Pamela; Luconi, Michaela. - In: ONCOTARGET. - ISSN 1949-2553. - STAMPA. - 8:(2017), pp. 65525-65533.. [10.18632/oncotarget.19118]

NEW INSIGHTS IN THE CLINICAL AND TRANSLATIONAL RELEVANCE OF miR483-5p IN ADRENOCORTICAL CANCER

SALVIANTI, FRANCESCA;CANU, LETIZIA;POLI, GIADA;ARMIGNACCO, ROBERTA;CANTINI, GIULIA;GELMINI, STEFANIA;ERCOLINO, TONINO;PAZZAGLI, MARIO;NESI, GABRIELLA;MANNELLI, MASSIMO;PINZANI, PAMELA;LUCONI, MICHAELA
2017

Abstract

Adrenocortical cancer (ACC) is a rare aggressive malignancy. Recent ACC integrated genomics analysis contributed to redefine the risk groups on molecular basis, including tumor microRNAs (miRs), detectable also in the bloodstream. We developed a quantitative real-time (RT) assay for the measurement of miR483 and miR483-5p absolute levels in plasma samples. miR483/miR483-5p levels were evaluated in plasma samples of 27 patients with ACC before surgery and at follow-up. Statistically significant differences in miR483-5p and miR483 levels were found between stage 1/2 and stage 3/4 ACCs in pre-surgery and post-surgery samples. ROC curve analysis of miR483–5p levels gave a prediction of the clinical stage (accuracy 0.917±0.084), with the best cut-off value of 0.221 ng/ml, prognosticating overall and recurrence-free survival. In a multivariate Cox analysis (HR 16.2, 95%CI[1.39-188.6, P<0.026]). miR483-5p was the only variable that significantly predicted recurrence, but not overall survival, In addition, miR483 and miR483-5p levels correlated with the number of circulating tumor cells (CTCs) detected in the same blood samples, independently of the timing of sampling. In conclusion, we demonstrated that miR483-5p absolute plasma levels in ACC patients are powerful molecular markers that may help in the follow-up of patients after surgery and chemotherapy, and contribute to more accurately classify and predict tumor progression.
2017
8
65525
65533.
Salvianti, Francesca; Canu, Letizia; Poli, Giada; Armignacco, Roberta; Scatena, Cristian; Cantini, Giulia; Franco, Alessandra Di; Gelmini, Stefania; Ercolino, Tonino; Pazzagli, Mario; Nesi, Gabriella; Mannelli, Massimo; Pinzani, Pamela; Luconi, Michaela
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1081354
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