Electrochemical immunoassay for Mucin 1 detection as a diagnostic tool in ovarian cancer

The immunoassay developed for Mucin 1 (MUC1) detection is based on a sandwich format in which a primary antibody immobilized on the surface of magnetic beads specifically binds the MUC1 protein. The sandwich immunoassay is performed by adding secondary anti-MUC1 and an alkaline-phosphatase-labeled third antibody. After, the modified magnetic beads are captured by a magnet on the surface of a graphite working electrode, and the electrochemical detection is thus achieved through the addition of 1-naphthyl phosphate. 1-naphthol, produced during the enzymatic reaction, is detected using differential pulse voltammetry (DPV). The performance of the assay in terms of sensitivity, reproducibility, and selectivity has been studied. MUC1 detection is performed in buffered solutions and serum sample.