This thesis describes the development of new kind of biosensors based on bio-mimetic probe molecules. Biosensor development can be classified as an interdisciplinary field that is one of the most active research areas in analytical chemistry. As well as others analytical methods, biosensors’ performances are evaluated considering their detection limit (LOD), their sensitivity, selectivity and reproducibility, the obtained linear and dynamic range and their response to interfering substance. Probably most used receptors in biosensing applications are Antibodies (ABs). They are able to bind the target providing high selectivity and sensitivity but their use is characterised by some limitations. Recent progresses in bio-analytical applications led to the synthesis and characterisation of new classes of biomimetic receptors. These kinds of probes are composed by biological “bricks” assembled in vitro or by synthetic molecules assembled in order to mimic ABs recognition capability. This thesis work will provide examples of applications of DNA aptamers and molecularly imprinted polymers (MIPs).
Improvements of sensing using synthetic bio-mimetic receptors / Rapini, Riccardo. - (2017).
Improvements of sensing using synthetic bio-mimetic receptors
RAPINI, RICCARDO
2017
Abstract
This thesis describes the development of new kind of biosensors based on bio-mimetic probe molecules. Biosensor development can be classified as an interdisciplinary field that is one of the most active research areas in analytical chemistry. As well as others analytical methods, biosensors’ performances are evaluated considering their detection limit (LOD), their sensitivity, selectivity and reproducibility, the obtained linear and dynamic range and their response to interfering substance. Probably most used receptors in biosensing applications are Antibodies (ABs). They are able to bind the target providing high selectivity and sensitivity but their use is characterised by some limitations. Recent progresses in bio-analytical applications led to the synthesis and characterisation of new classes of biomimetic receptors. These kinds of probes are composed by biological “bricks” assembled in vitro or by synthetic molecules assembled in order to mimic ABs recognition capability. This thesis work will provide examples of applications of DNA aptamers and molecularly imprinted polymers (MIPs).File | Dimensione | Formato | |
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PhD thesis_Riccardo Rapini.pdf
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Descrizione: tesi di dottorato Riccardo Rapini
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