Here we present development of optical techniques for the study of single processive myosin motors based on the combination of high-speed optical tweezers force spectroscopy and single molecule fluorescence imaging. Ultrafast force-clamp spectroscopy1 is applied to study the dependence of single chemo-mechanical steps of processive myosin motors on the applied load. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy)2, 3 is applied to in vitro motility assay to measure parameters such as the runlength, velocity and step size of single myosin V motors, labeled with Quantum Dots, under unloaded conditions.

Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy / Gardini, L.; Pavone, F. S.; Capitanio, M.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 10071:(2017), pp. 0-0. (Intervento presentato al convegno Single Molecule Spectroscopy and Superresolution Imaging X tenutosi a usa nel 2017) [10.1117/12.2256564].

Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy

Gardini, L.;Pavone, F. S.;Capitanio, M.
2017

Abstract

Here we present development of optical techniques for the study of single processive myosin motors based on the combination of high-speed optical tweezers force spectroscopy and single molecule fluorescence imaging. Ultrafast force-clamp spectroscopy1 is applied to study the dependence of single chemo-mechanical steps of processive myosin motors on the applied load. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy)2, 3 is applied to in vitro motility assay to measure parameters such as the runlength, velocity and step size of single myosin V motors, labeled with Quantum Dots, under unloaded conditions.
2017
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Single Molecule Spectroscopy and Superresolution Imaging X
usa
2017
Gardini, L.; Pavone, F. S.; Capitanio, M.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1104551
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact