β-catenin plays a dual role as a major constituent of cadherin-based adherens junctions and also as a transcriptional coactivator. In normal ephitelial cells, at adherens junction level, β-catenin links cadherins to the actin cytoskeleton. The structure of adherens junctions is dynamically regulated by tyrosine phosphorylation. In particular, cell-cell adhesion can be negatively regulated through the tyrosine phosphorylation of β-catenin. Furthermore, the loss of β-catenin-cadherin association has been correlated with the transition from a benign tumor to an invasive, metastatic cancer. Low-molecular-weight protein tyrosine phosphatase (LMW-PTP) is a ubiquitous PTP implicated in the regulation of mitosis and cytoskeleton rearrangement. Here we demonstrate that the amount of free cytoplasmic β-catenin is decreased in NIH3T3, which overexpresses active LMW-PTP, and this results in a stronger association between cadherin complexes and the actin-based cytoskeleton with respect to control cells. Confocal microscopy analysis shows that β-catenin colocalizes with LMW-PTP at the plasmamembrane. Furthermore, we provide evidence that β-catenin is able to associate with LMW-PTP both in vitro and in vivo. Moreover, overexpression of active LMW-PTP strongly potentiates cadherin-mediated cell-cell adhesion, whereas a dominant-negative form of LMW-PTP induces the opposite phenotype, both in NIH3T3 and in MCF-7 carcinoma cells. On the basis of these results, we propose that the stability of cell-cell contacts at the adherens junction level is positively influenced by LMW-PTP expression, mainly because of the β-catenin and LMW-PTP interaction at the plasmamembrane level with consequent dephosphorylation.

Beta-catenin interacts with low-molecular-weight protein tyrosine phosphatase leading to cadherin-mediated cell-cell adhesion increase / ML. TADDEI ; P. CHIARUGI ; P. CIRRI; F. BURICCHI ; T. FIASCHI ; E. GIANNONI ; D. TALINI ; G. COZZI ; L. FORMIGLI ; G. RAUGEI ; G. RAMPONI. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 62:(2002), pp. 6489-6499.

Beta-catenin interacts with low-molecular-weight protein tyrosine phosphatase leading to cadherin-mediated cell-cell adhesion increase.

TADDEI, MARIA LETIZIA;CHIARUGI, PAOLA;CIRRI, PAOLO;FIASCHI, TANIA;GIANNONI, ELISA;FORMIGLI, LUCIA;RAUGEI, GIOVANNI;RAMPONI, GIAMPIETRO
2002

Abstract

β-catenin plays a dual role as a major constituent of cadherin-based adherens junctions and also as a transcriptional coactivator. In normal ephitelial cells, at adherens junction level, β-catenin links cadherins to the actin cytoskeleton. The structure of adherens junctions is dynamically regulated by tyrosine phosphorylation. In particular, cell-cell adhesion can be negatively regulated through the tyrosine phosphorylation of β-catenin. Furthermore, the loss of β-catenin-cadherin association has been correlated with the transition from a benign tumor to an invasive, metastatic cancer. Low-molecular-weight protein tyrosine phosphatase (LMW-PTP) is a ubiquitous PTP implicated in the regulation of mitosis and cytoskeleton rearrangement. Here we demonstrate that the amount of free cytoplasmic β-catenin is decreased in NIH3T3, which overexpresses active LMW-PTP, and this results in a stronger association between cadherin complexes and the actin-based cytoskeleton with respect to control cells. Confocal microscopy analysis shows that β-catenin colocalizes with LMW-PTP at the plasmamembrane. Furthermore, we provide evidence that β-catenin is able to associate with LMW-PTP both in vitro and in vivo. Moreover, overexpression of active LMW-PTP strongly potentiates cadherin-mediated cell-cell adhesion, whereas a dominant-negative form of LMW-PTP induces the opposite phenotype, both in NIH3T3 and in MCF-7 carcinoma cells. On the basis of these results, we propose that the stability of cell-cell contacts at the adherens junction level is positively influenced by LMW-PTP expression, mainly because of the β-catenin and LMW-PTP interaction at the plasmamembrane level with consequent dephosphorylation.
2002
62
6489
6499
ML. TADDEI ; P. CHIARUGI ; P. CIRRI; F. BURICCHI ; T. FIASCHI ; E. GIANNONI ; D. TALINI ; G. COZZI ; L. FORMIGLI ; G. RAUGEI ; G. RAMPONI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/205811
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