Lung macrophages may play a relevant role in oxidative processes producing both superoxide anion O2 and NO. In this view,an antioxidant therapy can be useful in the treatment of systemic sclerosis (SSc) patients. N-Acetylcysteine (NAC) is able to expandnatural antioxidant defenses by increasing intracellular gluthatione concentration and it has been proposed as an antioxidant therapy in respiratory distress syndromes. The aim of our study was to determine whether lung macrophages obtained from SSc patient bronchoalveolar lavage (BAL) express the inducible form of nitric oxide synthase (iNOS) and whether NAC can reduce the peroxynitrite ONOO and O2 production of these cells. Alveolar macrophages were isolated from BAL of 32 patients and used for the immunocytochemical determination of iNOS, and the production of ONOO and O2 was measured by fluorimetric or spectrophotometric methods, respectively. Lung macrophages obtained from SSc patients expressed a higher level of iNOS compared to healthy subject cells. NAC preincubation (24 h) significantly reduced ()21%) the ONOO production in formyl Met-Leu-Phe (fMLP)-activated cells and slightly reduced it under resting conditions, whereas NAC preincubation was unable to modify the release of O2 both in basal condition and in fMLP-stimulated cells. We conclude that since SSc lung macrophages express high levels of iNOS and produce a significant quantity of ONOO, NAC administration reduces ONOO production and can be an useful treatment to alleviate SSc symptoms.

Effect of N-acetyl-L-cysteine on peroxynitrite and superoxide anion production of lung alveolar macrophages in systemic sclerosis / P. FAILLI; PALMIERI L.; D’ALFONSO C.; GIOVANNELLI L.; GENERINI S.; DEL ROSSO A.; PIGNONE A.; STANFLIN N.; ORSI S.; ZILLETTI L.; MATUCCI-CERINIC M.. - In: NITRIC OXIDE. - ISSN 1089-8603. - ELETTRONICO. - 7:(2002), pp. 277-282.

Effect of N-acetyl-L-cysteine on peroxynitrite and superoxide anion production of lung alveolar macrophages in systemic sclerosis

FAILLI, PAOLA;GIOVANNELLI, LISA;GENERINI, SERGIO;DEL ROSSO, ANGELA;PIGNONE A.;MATUCCI CERINIC, MARCO
2002

Abstract

Lung macrophages may play a relevant role in oxidative processes producing both superoxide anion O2 and NO. In this view,an antioxidant therapy can be useful in the treatment of systemic sclerosis (SSc) patients. N-Acetylcysteine (NAC) is able to expandnatural antioxidant defenses by increasing intracellular gluthatione concentration and it has been proposed as an antioxidant therapy in respiratory distress syndromes. The aim of our study was to determine whether lung macrophages obtained from SSc patient bronchoalveolar lavage (BAL) express the inducible form of nitric oxide synthase (iNOS) and whether NAC can reduce the peroxynitrite ONOO and O2 production of these cells. Alveolar macrophages were isolated from BAL of 32 patients and used for the immunocytochemical determination of iNOS, and the production of ONOO and O2 was measured by fluorimetric or spectrophotometric methods, respectively. Lung macrophages obtained from SSc patients expressed a higher level of iNOS compared to healthy subject cells. NAC preincubation (24 h) significantly reduced ()21%) the ONOO production in formyl Met-Leu-Phe (fMLP)-activated cells and slightly reduced it under resting conditions, whereas NAC preincubation was unable to modify the release of O2 both in basal condition and in fMLP-stimulated cells. We conclude that since SSc lung macrophages express high levels of iNOS and produce a significant quantity of ONOO, NAC administration reduces ONOO production and can be an useful treatment to alleviate SSc symptoms.
2002
7
277
282
P. FAILLI; PALMIERI L.; D’ALFONSO C.; GIOVANNELLI L.; GENERINI S.; DEL ROSSO A.; PIGNONE A.; STANFLIN N.; ORSI S.; ZILLETTI L.; MATUCCI-CERINIC M.
File in questo prodotto:
File Dimensione Formato  
Failli2002.pdf

accesso aperto

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: Open Access
Dimensione 166.23 kB
Formato Adobe PDF
166.23 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/208606
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact