Streptomyces sp A21 is a cellulolytic strain isolated from soil which was assigned to the genus Streptomyces on the basis of distinctive morphological features. A genomic library of A21 DNA has been constructed and transformed into Escherichia coli K-12 using a high-copy-number vector. One of the recombi- nant plasmids activates the cryptic bgl operon when in- serted into appropriate strains. The complete sequence of the 1629-bp A21 DNA fragment has been deter- mined. The analysis revealed the presence of an ORF whose putative product shows a high degree of similarity to RNA polymerase sigma factors; we therefore desig- nated the gene psfS (Putative sigma factor, Strepto- myces). Mapping of the 5¢ terminus of transcript by primer extension indicated that PsfS induces transcrip- tion initiation within the bgl promoter-silencer region.

A putative sigma factor from Streptomyces sp. strain A21 can activate the expression of the cryptic operon bgl in Escherichia coli K-12 / L.MARRI; S.DALY; B.PERITO; E.TAMBURINI; G.MASTROMEI. - In: MOLECULAR AND GENERAL GENETICS. - ISSN 0026-8925. - STAMPA. - 261:(1999), pp. 201-207.

A putative sigma factor from Streptomyces sp. strain A21 can activate the expression of the cryptic operon bgl in Escherichia coli K-12

PERITO, BRUNELLA;MASTROMEI, GIORGIO
1999

Abstract

Streptomyces sp A21 is a cellulolytic strain isolated from soil which was assigned to the genus Streptomyces on the basis of distinctive morphological features. A genomic library of A21 DNA has been constructed and transformed into Escherichia coli K-12 using a high-copy-number vector. One of the recombi- nant plasmids activates the cryptic bgl operon when in- serted into appropriate strains. The complete sequence of the 1629-bp A21 DNA fragment has been deter- mined. The analysis revealed the presence of an ORF whose putative product shows a high degree of similarity to RNA polymerase sigma factors; we therefore desig- nated the gene psfS (Putative sigma factor, Strepto- myces). Mapping of the 5¢ terminus of transcript by primer extension indicated that PsfS induces transcrip- tion initiation within the bgl promoter-silencer region.
1999
261
201
207
L.MARRI; S.DALY; B.PERITO; E.TAMBURINI; G.MASTROMEI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/217057
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