ABSTRACT Vascular endothelial cells have been identified as a source of substance P (SP) which may act in an autocrine/paracrine fashion to bring about nitric oxide (NO)-dependent vasodilatation and mitogen-induced cell division or immunologic and inflammatory responses. Whilst SP is localised in and released from endothelial cells, an endothelial mRNA expression of SP has not previously been shown. In the present study, mRNA expression of SP in human dermal microvascular endothelial cells is demonstrated using in situ hybridisation techniques with enhancement procedures. Incubation of microvascular endothelial cells with nerve growth factor (NGF) under conditions of increased shear stress increases the mRNA expression and release of SP. Endothelin (ET) release is also enhanced. These changes are pertinent to circulatory events that may occur in Raynaud’s phenomenon in systemic sclerosis

Regulation of substance P mRNA expression in human dermal microvascular endothelial cells / MILNER P; BODIN P; GUIDUCCI S; DEL ROSSO A; KAHALEH MB; MATUCCI CERINIC M; BURNSTOCK G.. - In: CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. - ISSN 0392-856X. - STAMPA. - 22(3 Suppl 33):(2004), pp. 24-27.

Regulation of substance P mRNA expression in human dermal microvascular endothelial cells.

GUIDUCCI, SERENA;DEL ROSSO, ANGELA;MATUCCI CERINIC, MARCO;
2004

Abstract

ABSTRACT Vascular endothelial cells have been identified as a source of substance P (SP) which may act in an autocrine/paracrine fashion to bring about nitric oxide (NO)-dependent vasodilatation and mitogen-induced cell division or immunologic and inflammatory responses. Whilst SP is localised in and released from endothelial cells, an endothelial mRNA expression of SP has not previously been shown. In the present study, mRNA expression of SP in human dermal microvascular endothelial cells is demonstrated using in situ hybridisation techniques with enhancement procedures. Incubation of microvascular endothelial cells with nerve growth factor (NGF) under conditions of increased shear stress increases the mRNA expression and release of SP. Endothelin (ET) release is also enhanced. These changes are pertinent to circulatory events that may occur in Raynaud’s phenomenon in systemic sclerosis
2004
22(3 Suppl 33)
24
27
MILNER P; BODIN P; GUIDUCCI S; DEL ROSSO A; KAHALEH MB; MATUCCI CERINIC M; BURNSTOCK G.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/217174
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