Cinta senese is an autochthonous pig breed native of Tuscany. The breed currently produces fresh and cured products with high market price. One of the factors associated with this high price is the origin of the raw material but consumers require that Cinta senese pig be the only breed involved. Microsatellite markers could help in breed identification and the first step to achieve this objective is the knowledge of the genetic variability. The purpose of the present work was to determine the genetic variability of Cinta Senese (CS) breed with ten microsatellites (SW2038, SW024, SW0017, SW153, SW1370, SW1035, SW1695, SW1873, SW1823 and SW1556) using Large White (LW) breed as reference. Genetic characterization was performed on 52 and 14 subjects for Cinta senese and Large white respectively. The following statistics were calculated: allele frequencies; mean heterozygosity values; average expected heterozygosity; coefficient of gene differentiation. The analysis of microsatellite loci revealed observed heterozygosities ranging from 0.31 to 0.92. The expected heterozygosity ranges from 0.444 to 0.892. A large part of the total genetic diversity can be explained by the variation within breeds (94.2%) and to a smaller extent by the variation among breeds although a large difference has been found among loci with Gst values ranging from 0.3% to 12.57%. Even if the mean number of alleles is similar between the two breeds (5.1), Cinta senese breed shows an almost unique pattern with many specific alleles and this is a good release point to setup a protocol for breed identification.
Preliminary analysis on genetic diversity of Cinta Senese pigs / R. BOZZI; A. CROVETTI; P. DEGLINNOCENTI; M. SAVARESE; A. VALENTINI; O. FRANCI. - STAMPA. - (2006), pp. 31-31. (Intervento presentato al convegno the Pig Genome I Symposium tenutosi a Lodi nel 20-21 febbraio).
Preliminary analysis on genetic diversity of Cinta Senese pigs
BOZZI, RICCARDO;CROVETTI, ALESSANDRO;FRANCI, ORESTE
2006
Abstract
Cinta senese is an autochthonous pig breed native of Tuscany. The breed currently produces fresh and cured products with high market price. One of the factors associated with this high price is the origin of the raw material but consumers require that Cinta senese pig be the only breed involved. Microsatellite markers could help in breed identification and the first step to achieve this objective is the knowledge of the genetic variability. The purpose of the present work was to determine the genetic variability of Cinta Senese (CS) breed with ten microsatellites (SW2038, SW024, SW0017, SW153, SW1370, SW1035, SW1695, SW1873, SW1823 and SW1556) using Large White (LW) breed as reference. Genetic characterization was performed on 52 and 14 subjects for Cinta senese and Large white respectively. The following statistics were calculated: allele frequencies; mean heterozygosity values; average expected heterozygosity; coefficient of gene differentiation. The analysis of microsatellite loci revealed observed heterozygosities ranging from 0.31 to 0.92. The expected heterozygosity ranges from 0.444 to 0.892. A large part of the total genetic diversity can be explained by the variation within breeds (94.2%) and to a smaller extent by the variation among breeds although a large difference has been found among loci with Gst values ranging from 0.3% to 12.57%. Even if the mean number of alleles is similar between the two breeds (5.1), Cinta senese breed shows an almost unique pattern with many specific alleles and this is a good release point to setup a protocol for breed identification.
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