S-phenylmercapturic acid (PMA) is a specific urinary biomarker of benzene at exposure levels lower than 1 ppm. However, measuring PMA in urine is an expensive task by either GC or HPLC due to the necessity of extensive sample pretreatment. In the present study, a commercial chemiluminescence enzyme-linked immunosorbent assay (ELISA) test for PMA and GC-MS were used for screening urine samples of 60 workers employed in petrochemical settings. The ELISA results were evaluated by comparison with the GC-MS. Overall, the ELISA test proved sensitive (limit of detection/0.1 mg l1), rapid, robust and reliable, affording results in good agreement with the GC-MS (54% of measurements) and no false-negatives. On the other hand, 46% of the ELISA assays were assigned as false-positives (arbitrarily established when ELISA /5 mg l1, GC-MS B/5 mg l1) and a correlation coefficient of 0.687 was calculated between the two methods. It appears that urinary PMA routine biomonitoring on large numbers of samples is carried out in a cost-effective and rapid approach by preliminary screening with the ELISA assay followed by GC-MS confirmation of concentrations exceeding the biological exposure index for PMA.

Determination of S-phenylmercapturic acid by GC-MS and ELISA: a comparison of the two methods / MARRUBINI G; DUGHERI S; PACENTI M; COCCINI T; ARCANGELI G; V. CUPELLI; MANZO L. - In: BIOMARKERS. - ISSN 1354-750X. - ELETTRONICO. - 10:(2005), pp. 238-251.

Determination of S-phenylmercapturic acid by GC-MS and ELISA: a comparison of the two methods.

PACENTI, MARCO;ARCANGELI, GIULIO;CUPELLI, VINCENZO;
2005

Abstract

S-phenylmercapturic acid (PMA) is a specific urinary biomarker of benzene at exposure levels lower than 1 ppm. However, measuring PMA in urine is an expensive task by either GC or HPLC due to the necessity of extensive sample pretreatment. In the present study, a commercial chemiluminescence enzyme-linked immunosorbent assay (ELISA) test for PMA and GC-MS were used for screening urine samples of 60 workers employed in petrochemical settings. The ELISA results were evaluated by comparison with the GC-MS. Overall, the ELISA test proved sensitive (limit of detection/0.1 mg l1), rapid, robust and reliable, affording results in good agreement with the GC-MS (54% of measurements) and no false-negatives. On the other hand, 46% of the ELISA assays were assigned as false-positives (arbitrarily established when ELISA /5 mg l1, GC-MS B/5 mg l1) and a correlation coefficient of 0.687 was calculated between the two methods. It appears that urinary PMA routine biomonitoring on large numbers of samples is carried out in a cost-effective and rapid approach by preliminary screening with the ELISA assay followed by GC-MS confirmation of concentrations exceeding the biological exposure index for PMA.
2005
10
238
251
MARRUBINI G; DUGHERI S; PACENTI M; COCCINI T; ARCANGELI G; V. CUPELLI; MANZO L
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/251216
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