Hepatitis C virus (HCV) core protein has been shown to deregulate cell growth and programmed cell death in hepatoma cells, but only minimal informations are available about its possible role on B-lymphoproliferative disorders (LPDs). The aim of our work was to analyze the biological activity ofHCVcore protein on B-cell proliferation.Weestablished Wil2-ns and Ramos B-cell lines that stably expressed the HCVcore protein. Growth curve, thymidine incorporation analysis, as well as the expression ofPCNA and activated-ERKs demonstrated that HCV core protein induced an increased growth in both cell lines. Interestingly, the HCV core protein expression determined, in our model, a downregulation of DNp73 and an upregulation of DNp63, which was essential for the maintenance of viral-dependent effects on cell growth. Finally, we have identified phosphoinositide 3-kinase (PI3K) as mediator of HCV core-dependent transcriptional increase of DNp63, which in turn correlated with the increasing of lymphocyte proliferation. In primary B-lymphocytes, derived from HCV-related low-grade non-Hodgkin’s lymphoma patients, consistent results were obtained. These findings provide evidence for a possible pathogenetic role played by HCV core protein in HCV-related lymphomagenesis; it could occur through the deregulation of PI3K activity, consequent activation of Akt and overexpression of DNp63.

Involvement of PI3K in HCV-related lymphoproliferative disorders / A. Alisi; C. Giannini; A. Spaziani; P. Caini ; A.L. Zignego; C. Balsano. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 214(2):(2008), pp. 396-404.

Involvement of PI3K in HCV-related lymphoproliferative disorders

GIANNINI, CARLO;CAINI, PATRIZIO;ZIGNEGO, ANNA LINDA;
2008

Abstract

Hepatitis C virus (HCV) core protein has been shown to deregulate cell growth and programmed cell death in hepatoma cells, but only minimal informations are available about its possible role on B-lymphoproliferative disorders (LPDs). The aim of our work was to analyze the biological activity ofHCVcore protein on B-cell proliferation.Weestablished Wil2-ns and Ramos B-cell lines that stably expressed the HCVcore protein. Growth curve, thymidine incorporation analysis, as well as the expression ofPCNA and activated-ERKs demonstrated that HCV core protein induced an increased growth in both cell lines. Interestingly, the HCV core protein expression determined, in our model, a downregulation of DNp73 and an upregulation of DNp63, which was essential for the maintenance of viral-dependent effects on cell growth. Finally, we have identified phosphoinositide 3-kinase (PI3K) as mediator of HCV core-dependent transcriptional increase of DNp63, which in turn correlated with the increasing of lymphocyte proliferation. In primary B-lymphocytes, derived from HCV-related low-grade non-Hodgkin’s lymphoma patients, consistent results were obtained. These findings provide evidence for a possible pathogenetic role played by HCV core protein in HCV-related lymphomagenesis; it could occur through the deregulation of PI3K activity, consequent activation of Akt and overexpression of DNp63.
2008
214(2)
396
404
A. Alisi; C. Giannini; A. Spaziani; P. Caini ; A.L. Zignego; C. Balsano
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/257411
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