DNA fingerprints of several Azospirillum strains, belonging to the five known species A. amuwnense, A. brasilense, A. halopraeferens, A. irakense and A. lipoferum, were obtained by restriction analysis of the amplified 16s rDNA and by restriction fragment length polymorphism of the histidiue biosynthetic genes. Data obtained showed that amplified rDNA restriction analysis is an easy, fast, reproducible and reliable tool for identification of Azospirillum strains, mainly at the species level, whereas restriction fragment length polymorphism could, in some cases, differentiate strains belonging to the same species. Moreover, both analyses gave congruent results in grouping strains and in the assignment of new strains to a given species.
PHYLOGENY OF THE GENUS AZOSPIRILLUM BASED ON 16S RDNA SEQUENCES / R. FANI; M. BAZZICALUPO; M. CECCHERINI; S. FANCELLI; E. GALLORI; A. GRIFONI; G. DAMIANI; N. MICLAUS; C. BANDI; L. GERACE. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - STAMPA. - 129:(1995), pp. 195-200.
PHYLOGENY OF THE GENUS AZOSPIRILLUM BASED ON 16S RDNA SEQUENCES
FANI, RENATO;BAZZICALUPO, MARCO;CECCHERINI, MARIA TERESA;GALLORI, ENZO;
1995
Abstract
DNA fingerprints of several Azospirillum strains, belonging to the five known species A. amuwnense, A. brasilense, A. halopraeferens, A. irakense and A. lipoferum, were obtained by restriction analysis of the amplified 16s rDNA and by restriction fragment length polymorphism of the histidiue biosynthetic genes. Data obtained showed that amplified rDNA restriction analysis is an easy, fast, reproducible and reliable tool for identification of Azospirillum strains, mainly at the species level, whereas restriction fragment length polymorphism could, in some cases, differentiate strains belonging to the same species. Moreover, both analyses gave congruent results in grouping strains and in the assignment of new strains to a given species.
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