The structure of the metal-free form of CopC, a protein involved in copper homeostasis, has been obtained. The fold is a Greek key beta barrel similar to that of functionally unrelated blue copper proteins but with important structural variations. The protein binds one equivalent of copper (II) with relatively high affinity and contains a cluster of conserved residues (His1, Glu27, Asp89, and His91) which could form a water-accessible metal binding site. The structure also reveals a loop containing the M(X)(n)M motif which is present in a number of proteins also involved in copper homeostasis. The present structure represents a link between copper-trafficking proteins and cupredoxins. Within a structural and genomic analysis, the role of CopC in copper trafficking is discussed.

Solution structure of CopC: a cupredoxin-like protein involved in copper homeostasis / I. BERTINI; L. BANCI; A. THOMPSETT; F. ARNESANO. - In: STRUCTURE. - ISSN 0969-2126. - STAMPA. - 10:(2002), pp. 1337-1347. [10.1016/S0969-2126(02)00858-4]

Solution structure of CopC: a cupredoxin-like protein involved in copper homeostasis

BERTINI, IVANO;BANCI, LUCIA;
2002

Abstract

The structure of the metal-free form of CopC, a protein involved in copper homeostasis, has been obtained. The fold is a Greek key beta barrel similar to that of functionally unrelated blue copper proteins but with important structural variations. The protein binds one equivalent of copper (II) with relatively high affinity and contains a cluster of conserved residues (His1, Glu27, Asp89, and His91) which could form a water-accessible metal binding site. The structure also reveals a loop containing the M(X)(n)M motif which is present in a number of proteins also involved in copper homeostasis. The present structure represents a link between copper-trafficking proteins and cupredoxins. Within a structural and genomic analysis, the role of CopC in copper trafficking is discussed.
2002
10
1337
1347
I. BERTINI; L. BANCI; A. THOMPSETT; F. ARNESANO
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/312216
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