This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe ( SPINE) consortium. Focusing on the development of high- throughput ( HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless C-13-direct detection methods and inferential structure determinations ( ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations
NMR in the SPINE Structural Proteomics project / E.Ab; A.R.Atkinson; L.Banci; I.Bertini; S.Ciofi-Baffoni; K.Brunner; T.Diercks; V.Doetsch; F.Engelke; G.E.Folkers; C.Griesinger; W.Gronwald; H.Guenther; M.Habeck; R.de Jong; H.R.Kalbitzer; B.Kieffer; B.R.Leeflang; S.Loss; C.Luchinat; T.Marquardsen; D.Moskau; K.P.Neidig; M.Nilges; M.Piccioli; R.Pierattelli; W.Rieping; T.Schippmann; H.Schwalbe; G.Travé; J.M.Trenner; J.Wohnert; M.Zweckstetter; R.Kaptein. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - STAMPA. - 62:(2006), pp. 1150-1161. [10.1107/S0907444906032070]
NMR in the SPINE Structural Proteomics project
BANCI, LUCIA;BERTINI, IVANO;CIOFI BAFFONI, SIMONE;LUCHINAT, CLAUDIO;PICCIOLI, MARIO;PIERATTELLI, ROBERTA;
2006
Abstract
This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe ( SPINE) consortium. Focusing on the development of high- throughput ( HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless C-13-direct detection methods and inferential structure determinations ( ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinationsI documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.