The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 mM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.

Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification / G. Camici; G. Manao; G. Ramponi. - In: PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR. - ISSN 0748-6642. - STAMPA. - 7:(1975), pp. 409-414.

Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification.

CAMICI, GUIDO;MANAO, GIAMPAOLO;RAMPONI, GIAMPIETRO
1975

Abstract

The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 mM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
1975
7
409
414
G. Camici; G. Manao; G. Ramponi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/323517
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