HPLC characterization of polyphenols and oligosaccharides in Phaseolus vulgaris L. Italian landraces

A simple extraction procedure was optimized to characterize and quantify the polyphenols and oligosaccharides of whole seeds of four landraces of Phaseolus vulgaris L., which have not previously been investigated. The samples were collected in different Italian regions (Tuscany and Basilicata) in three different years. Flavonols, isoflavones and hydroxycinnamic derivatives were detected and characterized by HPLC/DAD/MS. The total amount of polyphenols ranged from 10.69 to 1,218.93 mg/kg. Oligosaccharides (sucrose, raffinose and stachyose) were detected and identified by HPLC/RI. The total oligosaccharidic amount ranged from 7.738 to 5.081 g/100g of seed flour. Kaempferol derivatives, hydroxycinnamic derivatives, stachyose and sucrose contents differentiated three landraces at the 1% significance level.