The possible role of T lymphocytes in the formation of granulomatous reactions seen in certain malignant lymphoid tumours was investigated by measuring the granulopoietic colony‐stimulating activity (CSA) and granulopoietic‐inhibiting activity (IA) produced by stimulated T‐lymphocytes isolated from peripheral blood, spleen and lymph nodes of patients and normal subjects. Lymph‐node T‐cells from patients with benign lymphoid hyperplasia, B‐cell non‐Hodgkin's lymphoma (B‐NHL), and non‐granulomatous Hodgkin's disease (HD) showed no CSA, but the cells produced [A of 40 ± 23%, 40 ± 24% and 50·5 ± 22·5% respectively. The corresponding cells from patients with HD accompanied by granulomatous reactions produced CSA of 6·85 ± 6·5 u/μl and IA of 23·5 ± 21%. The presence of a granulomatous reaction in malignant lymphoma was correlated with the stimulation of granulopoiesis in vitro by T lymphocytes associated with malignant cells. A correlation was demonstrated between neutrophilic and eosinophilic colonies obtained in vitro under the influence of CSA‐producing T cells isolated from malignant lymphomas and the neutrophils and eosinophils present in the granuloma. These results showed that tumour‐infiltrating T cells play a role in the presence of granulomatous reactions seen in lymphomas. Peripheral‐blood T cells from healthy subjects, and from patients with B‐NHL, or with HD unaccompanied by granulocytic reactions produced CSAs of, respectively, 5 ± 0·5 u/μl, 4·8 ± 2·2 u/μl and 5·3 ± 0·4 u/μl, and IAs of 45 ± 18%, 50 ± 5·5% and 50·5 ± 7% respectively. The corresponding values for HD patients with granulocytic reactions were CSA, 17 ± 15·5 u/μl, and IA, 9·5 ± 9%. No correlation was demonstrated between neutrophilic colonies obtained in vitro under the influence of HD blood T cells and neutrophils present in blood. Only one correlation was found: between the percentage of eosinophilic colonies and the number of blood eosinophils. HD blood T cells did not seem to explain completely granulocytic reactions seen in blood.

PRODUCTION OF GRANULOPOIESIS-STIMULATING AND GRANULOPOIESIS-INHIBITING ACTIVITIES BY T-CELLS ASSOCIATED WITH MALIGNANT-CELLS IN LYMPHOMAS / Piccinni MP; Jacob MC; Bonnefoix T; Sotto MF; Pegourie B; Couderc P; Sotto JJ.. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - STAMPA. - 74:(1990), pp. 432-438.

PRODUCTION OF GRANULOPOIESIS-STIMULATING AND GRANULOPOIESIS-INHIBITING ACTIVITIES BY T-CELLS ASSOCIATED WITH MALIGNANT-CELLS IN LYMPHOMAS

PICCINNI, MARIE-PIERRE;
1990

Abstract

The possible role of T lymphocytes in the formation of granulomatous reactions seen in certain malignant lymphoid tumours was investigated by measuring the granulopoietic colony‐stimulating activity (CSA) and granulopoietic‐inhibiting activity (IA) produced by stimulated T‐lymphocytes isolated from peripheral blood, spleen and lymph nodes of patients and normal subjects. Lymph‐node T‐cells from patients with benign lymphoid hyperplasia, B‐cell non‐Hodgkin's lymphoma (B‐NHL), and non‐granulomatous Hodgkin's disease (HD) showed no CSA, but the cells produced [A of 40 ± 23%, 40 ± 24% and 50·5 ± 22·5% respectively. The corresponding cells from patients with HD accompanied by granulomatous reactions produced CSA of 6·85 ± 6·5 u/μl and IA of 23·5 ± 21%. The presence of a granulomatous reaction in malignant lymphoma was correlated with the stimulation of granulopoiesis in vitro by T lymphocytes associated with malignant cells. A correlation was demonstrated between neutrophilic and eosinophilic colonies obtained in vitro under the influence of CSA‐producing T cells isolated from malignant lymphomas and the neutrophils and eosinophils present in the granuloma. These results showed that tumour‐infiltrating T cells play a role in the presence of granulomatous reactions seen in lymphomas. Peripheral‐blood T cells from healthy subjects, and from patients with B‐NHL, or with HD unaccompanied by granulocytic reactions produced CSAs of, respectively, 5 ± 0·5 u/μl, 4·8 ± 2·2 u/μl and 5·3 ± 0·4 u/μl, and IAs of 45 ± 18%, 50 ± 5·5% and 50·5 ± 7% respectively. The corresponding values for HD patients with granulocytic reactions were CSA, 17 ± 15·5 u/μl, and IA, 9·5 ± 9%. No correlation was demonstrated between neutrophilic colonies obtained in vitro under the influence of HD blood T cells and neutrophils present in blood. Only one correlation was found: between the percentage of eosinophilic colonies and the number of blood eosinophils. HD blood T cells did not seem to explain completely granulocytic reactions seen in blood.
1990
74
432
438
Goal 3: Good health and well-being for people
Piccinni MP; Jacob MC; Bonnefoix T; Sotto MF; Pegourie B; Couderc P; Sotto JJ.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/336667
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