Quantification of orotic acid (uracil-6-carboxylic acid) in urine is an important tool to diagnose some inherited diseases, such as urea cycle disorder (OTCD) and hereditary orotic aciduria. New rapid analytical methods are necessary to provide gh-throughput orotic acid analyses. A new analytical method has been eveloped for the rapid analysis of orotic acid in urine by liquid chromatography coupled with ion spray tandem mass spectrometry (LC/MS/MS). After a sample dilution 1:20, the analysis was performed in the selected reaction monitoring mode in which orotic acid was detected through the transition m/z 155 to 111. The retention time was 3.9 min in a 4.5-min analysis. Daily calibration between 0.5–5.0mmol/L of orotic acid, corresponding to 10–100mmol/L in urine before the 1:20 dilution, offered consistent linearity and reproducibility. Interassay coefficient of variance (c.v.) was 4.97% at a mean concentration of 10.99mmol/L. The sensitivity and specificity of tandem mass spectrometry permitted a high volume of analyses of orotic acid. The sample preparation is simple, inexpensive and not time demanding.

Rapid determination of orotic acid in urine by a fast liquid chromatography/tandem mass spectrometric method / la Marca G; Casetta B; Zammarchi E. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - ELETTRONICO. - 17(8):(2003), pp. 788-793.

Rapid determination of orotic acid in urine by a fast liquid chromatography/tandem mass spectrometric method.

LA MARCA, GIANCARLO;ZAMMARCHI, ENRICO
2003

Abstract

Quantification of orotic acid (uracil-6-carboxylic acid) in urine is an important tool to diagnose some inherited diseases, such as urea cycle disorder (OTCD) and hereditary orotic aciduria. New rapid analytical methods are necessary to provide gh-throughput orotic acid analyses. A new analytical method has been eveloped for the rapid analysis of orotic acid in urine by liquid chromatography coupled with ion spray tandem mass spectrometry (LC/MS/MS). After a sample dilution 1:20, the analysis was performed in the selected reaction monitoring mode in which orotic acid was detected through the transition m/z 155 to 111. The retention time was 3.9 min in a 4.5-min analysis. Daily calibration between 0.5–5.0mmol/L of orotic acid, corresponding to 10–100mmol/L in urine before the 1:20 dilution, offered consistent linearity and reproducibility. Interassay coefficient of variance (c.v.) was 4.97% at a mean concentration of 10.99mmol/L. The sensitivity and specificity of tandem mass spectrometry permitted a high volume of analyses of orotic acid. The sample preparation is simple, inexpensive and not time demanding.
2003
17(8)
788
793
la Marca G; Casetta B; Zammarchi E
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/348779
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