BCL-2 is a membrane protein known to be an apoptosis inhibitor. It is the product of the bcl-2 gene located on chromosome 18. Several different tumors show BCL-2 over-expression as result of a translocation or independently from it. More than 85% of follicular lymphomas and a smaller number of diffuse large cell B lymphomas contain t(14;18) (q32;q2l). The aim of this study was to investigate the immunohistochemical expression of the BCL-2 protein and to ascertain, by means of traditional PCR (Polimerase Chain Reaction), its possible dependence from t(14;18) (q32;q2l) in 9 primary central nervous system lymphomas. Six cases (67%) shoved immunohistochemical BCL-2 over-expression and 3 cases (33%) had t(14;18). Precisely: 2 cases (22%) had immunohistochemical BCL-2 over-expression and t(14;18) (q32;q2l); 4 cases (44%) had BCL-2 over-expression without translocation; 1 case (11 %) did not show diffuse BCL-2 over-expression in presence of the traslocation; the remaining 2 cases (22%) did not demonstrate BCL-2 over-expression or t(14;18) (q32;q2l). In conclusion, our results indicate primary central nervous system lymphomas frequently show BCL-2 over-expression that in some case may be related to t(14;18) (q32;q2l). Nevertheless, t(14;18) (q32;q2l), as evaluated by traditional PCR, may not correspond to diffuse immunohistochemical BCL-2 positivity.

BCL-2 in primary central nervous system lymphomas. Immunohistochemistry and molecular biology / A. M. Buccoliero;F. Castiglione;A. Caldarella;D. R. Degl'Innocenti;A. Taddei;F. Ammannati;P. Mennonna;G. L. Taddei. - In: PATHOLOGICA. - ISSN 0031-2983. - STAMPA. - 96:(2004), pp. 425-429.

BCL-2 in primary central nervous system lymphomas. Immunohistochemistry and molecular biology

CASTIGLIONE, FRANCESCA;ROSSI DEGL'INNOCENTI, DUCCIO;TADDEI, ANTONIO;
2004

Abstract

BCL-2 is a membrane protein known to be an apoptosis inhibitor. It is the product of the bcl-2 gene located on chromosome 18. Several different tumors show BCL-2 over-expression as result of a translocation or independently from it. More than 85% of follicular lymphomas and a smaller number of diffuse large cell B lymphomas contain t(14;18) (q32;q2l). The aim of this study was to investigate the immunohistochemical expression of the BCL-2 protein and to ascertain, by means of traditional PCR (Polimerase Chain Reaction), its possible dependence from t(14;18) (q32;q2l) in 9 primary central nervous system lymphomas. Six cases (67%) shoved immunohistochemical BCL-2 over-expression and 3 cases (33%) had t(14;18). Precisely: 2 cases (22%) had immunohistochemical BCL-2 over-expression and t(14;18) (q32;q2l); 4 cases (44%) had BCL-2 over-expression without translocation; 1 case (11 %) did not show diffuse BCL-2 over-expression in presence of the traslocation; the remaining 2 cases (22%) did not demonstrate BCL-2 over-expression or t(14;18) (q32;q2l). In conclusion, our results indicate primary central nervous system lymphomas frequently show BCL-2 over-expression that in some case may be related to t(14;18) (q32;q2l). Nevertheless, t(14;18) (q32;q2l), as evaluated by traditional PCR, may not correspond to diffuse immunohistochemical BCL-2 positivity.
2004
96
425
429
A. M. Buccoliero;F. Castiglione;A. Caldarella;D. R. Degl'Innocenti;A. Taddei;F. Ammannati;P. Mennonna;G. L. Taddei
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/359930
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