Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid which regulates multiple biological parameters in a number of cell types, including stem cells.Here we report, for the first time, that S1P dose-dependently stimulates differentiation of adipose tissue-derivedmesenchymal stem cells (ASMC) towards smooth muscle cells. Indeed, S1P not only induced the expression of smooth muscle cell-specific proteins such as a-smooth muscle actin (aSMA) and transgelin, but also profoundly affected ASMC morphology by enhancing cytoskeletal F-actin assembly, which incorporated aSMA. More importantly, S1P challenge was responsible for the functional appearance of Ca2+ currents, characteristic of differentiated excitable cells such as smooth muscle cells. By employing various agonists and antagonists to inhibit S1P receptor subtypes, S1P2 turned out to be critical for the pro-differentiating effect of S1P, while S1P3 appeared to play a secondary role. This study individuates an important role of S1P in AMSC which can be exploited to favour vascular regeneration.

Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells / P.Nincheri; P.Luciani; R.Squecco; C.Donati; C.Bernacchioni; L.Borgognoni; G.Luciani; S.Benvenuti; F.Francini; P. Bruni. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - STAMPA. - 66:(2009), pp. 1741-1754.

Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.

NINCHERI, PAOLA;LUCIANI, PAOLA;SQUECCO, ROBERTA;DONATI, CHIARA;BERNACCHIONI, CATERINA;LUCIANI, GIORGIA;BENVENUTI, SUSANNA;FRANCINI, FABIO;BRUNI, PAOLA
2009

Abstract

Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid which regulates multiple biological parameters in a number of cell types, including stem cells.Here we report, for the first time, that S1P dose-dependently stimulates differentiation of adipose tissue-derivedmesenchymal stem cells (ASMC) towards smooth muscle cells. Indeed, S1P not only induced the expression of smooth muscle cell-specific proteins such as a-smooth muscle actin (aSMA) and transgelin, but also profoundly affected ASMC morphology by enhancing cytoskeletal F-actin assembly, which incorporated aSMA. More importantly, S1P challenge was responsible for the functional appearance of Ca2+ currents, characteristic of differentiated excitable cells such as smooth muscle cells. By employing various agonists and antagonists to inhibit S1P receptor subtypes, S1P2 turned out to be critical for the pro-differentiating effect of S1P, while S1P3 appeared to play a secondary role. This study individuates an important role of S1P in AMSC which can be exploited to favour vascular regeneration.
2009
66
1741
1754
P.Nincheri; P.Luciani; R.Squecco; C.Donati; C.Bernacchioni; L.Borgognoni; G.Luciani; S.Benvenuti; F.Francini; P. Bruni
File in questo prodotto:
File Dimensione Formato  
CMLS-stemcell.pdf

Accesso chiuso

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: Tutti i diritti riservati
Dimensione 2.31 MB
Formato Adobe PDF
2.31 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/360602
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 55
  • ???jsp.display-item.citation.isi??? ND
social impact