Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.

IgG and IgM antibodies to the refolded MOG(1-125) extracellular domain in humans / F. Gori;B. Mulinacci;L. Massai;C. Avolio;M. Caragnano;E. Peroni;S. Lori;M. Chelli; A.M. Papini; P. Rovero; F. Lolli. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 233:(2011), pp. 216-220. [10.1016/j.jneuroim.2010.11.011]

IgG and IgM antibodies to the refolded MOG(1-125) extracellular domain in humans.

PAPINI, ANNA MARIA;ROVERO, PAOLO;LOLLI, FRANCESCO
2011

Abstract

Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.
2011
233
216
220
F. Gori;B. Mulinacci;L. Massai;C. Avolio;M. Caragnano;E. Peroni;S. Lori;M. Chelli; A.M. Papini; P. Rovero; F. Lolli
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/405168
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