The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01–5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.

New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction couplet to fast gas chromatography/negative chemical-electron ionization/mass spectrometry / M. PACENTI;S. DUGHERI;P. TRALDI;M. CALAMANTE;I. KIKIC;P. ALESSI;A. BONACCHI;E. SALVADORI;G. ARCANGELI;V. CUPELLI; F. DEGLI ESPOSTI; N. PERCHIAZZI; E. FRANCHI. - In: JOURNAL OF AUTOMATED METHODS & MANAGEMENT IN CHEMISTRY. - ISSN 1463-9246. - STAMPA. - 61:(2010), pp. 1-13. [10.1155/2010/972926]

New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction couplet to fast gas chromatography/negative chemical-electron ionization/mass spectrometry.

G. ARCANGELI;V. CUPELLI;
2010

Abstract

The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01–5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.
2010
61
1
13
M. PACENTI;S. DUGHERI;P. TRALDI;M. CALAMANTE;I. KIKIC;P. ALESSI;A. BONACCHI;E. SALVADORI;G. ARCANGELI;V. CUPELLI; F. DEGLI ESPOSTI; N. PERCHIAZZI; E. FRANCHI
File in questo prodotto:
File Dimensione Formato  
New Automated and High-Throughput Quantitative Analysis of Urinary Ketones.pdf

Accesso chiuso

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: Tutti i diritti riservati
Dimensione 1.45 MB
Formato Adobe PDF
1.45 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/406328
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 15
social impact