Increasing evidences suggest that oxidative stress play a critical role in the development of human heart failure. During pathophysiological conditions, the balance between free radicals and antioxidants may shift towards a relative increase of free radicals resulting in oxidative stress. Few and conflicting data are available on antioxidant defences in human failing hearts and they are limited to left (LV) ventricle. The aim of this study was to investigate the source of free radicals and antioxidant enzyme activities in the right (RV) and LV ventricles of human failing hearts and their mutual relationship. We found a significant increase in NADPH oxidase activity in both ventricles of failing hearts. Protein expression and catalytic activity of catalase (CAT), glutathione peroxidase (GPx), manganese superoxide dismutase (Mn-SOD) were also evaluated. Despite unchanged protein expression of all enzymes, significant enhances in GPx and CAT activity were observed. No change in Mn-SOD activity was detected. Interestingly, a significant correlation was found between the values of GPx and catalase activity in LV and RV of the same heart. In addition, an increase in NADPH oxidase-dependent superoxide production positively correlated with the activation of both antioxidant enzymes. Our findings suggest that a similar adaptive response occurs in LV and RV ventricles of failing hearts and support the hypothesis that the upregulation of antioxidant enzymes may be due to post-transductional modifications induced by an increased NADPH-oxidase superoxide generation.

Glutathione peroxidase and catalase activities increase in left and right ventricles of human failing heart: an adaptive response to oxidative stress / E. Borchi; V. Bargelli; C. Giordano; G. D'Amati; E. Cerbai; C. Nediani.. - STAMPA. - Abstracts Book:(2008), pp. 73-73. (Intervento presentato al convegno 16th JMRC Symposium tenutosi a Krakow, Poland nel 5th-7th June).

Glutathione peroxidase and catalase activities increase in left and right ventricles of human failing heart: an adaptive response to oxidative stress.

BORCHI, ELISABETTA;CERBAI, ELISABETTA;NEDIANI, CHIARA
2008

Abstract

Increasing evidences suggest that oxidative stress play a critical role in the development of human heart failure. During pathophysiological conditions, the balance between free radicals and antioxidants may shift towards a relative increase of free radicals resulting in oxidative stress. Few and conflicting data are available on antioxidant defences in human failing hearts and they are limited to left (LV) ventricle. The aim of this study was to investigate the source of free radicals and antioxidant enzyme activities in the right (RV) and LV ventricles of human failing hearts and their mutual relationship. We found a significant increase in NADPH oxidase activity in both ventricles of failing hearts. Protein expression and catalytic activity of catalase (CAT), glutathione peroxidase (GPx), manganese superoxide dismutase (Mn-SOD) were also evaluated. Despite unchanged protein expression of all enzymes, significant enhances in GPx and CAT activity were observed. No change in Mn-SOD activity was detected. Interestingly, a significant correlation was found between the values of GPx and catalase activity in LV and RV of the same heart. In addition, an increase in NADPH oxidase-dependent superoxide production positively correlated with the activation of both antioxidant enzymes. Our findings suggest that a similar adaptive response occurs in LV and RV ventricles of failing hearts and support the hypothesis that the upregulation of antioxidant enzymes may be due to post-transductional modifications induced by an increased NADPH-oxidase superoxide generation.
2008
Abstract Book of 16th JMRC Symposium "New Frontiers in Cardiovascular Research"
16th JMRC Symposium
Krakow, Poland
E. Borchi; V. Bargelli; C. Giordano; G. D'Amati; E. Cerbai; C. Nediani.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/406513
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