Shellfish are perishable products due to their biochemical composition. Under refrigerated storage conditions, the shelf life of these products is limited by microbiological and enzymatic spoilage. Aim of the work was the evaluation of the influence of culture site on quality and safety of oysters and mussels, during the post-harvest refrigerated storage. In this study, 145 oysters and 90 mussels, sampled in Orbetello Lagoon (Italy), at March and at September respectively, were monitored during the storage at 4°C. The shellfish were analysed at 1, 3, 7 and 10 days from the harvesting by counting of aerobic psychrotrophic bacterial, pseudomonads, coliform bacteria and Escherichia coli. In order to deepen the knowledge about the role of pseudomonads, the significant number of strains from oysters and mussels were isolated. Total DNA was extracted from the isolates and 16S rDNA was PCR-amplified using FD1 and RD1 primers. Amplicons were subjected to ARDRA analysis using the restriction endonuclease CfoI. Representative strains of ARDRA groups were identified by 16S rDNA sequencing. In oysters, the aerobic psychrotrophic bacterial counts was 4.5 and 6.0 log CFUg–1 on the 1st and the 10th day, respectively; the pseudomonads values was 3.2 and 5.3 log CFUg–1 on the 1st and the 10th day respectively. In mussels, the aerobic psychrotrophic bacterial count was 5.8 and 6.2 log CFUg–1 on the 1st and the 10th day, respectively; the pseudomonads value was 4.2 and 6.2 log CFUg–1 on the 1st and the 10th day respectively. The coliform number was always below the limit permitted by the Italian law and Escherichia coli was always undetected. 16S rDNA sequencing indicated that the analysed isolates (pseudomonads), from oysters and safety mussels, belong to Pseudomonas spp.. Oysters and mussels maintained appreciable quality and safety characteristics during ten days of refrigerated storage.

Quality and safety in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis) reared in Orbetello lagoon (Central Italy) / A.MESSINI; G.PARISI; G. FRATINI. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - STAMPA. - 10(1):(2011), pp. 111-111. (Intervento presentato al convegno XIX Congress of the Animal Science and Production Association A.S.P.A. tenutosi a Cremona nel 7-10 June 2011).

Quality and safety in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis) reared in Orbetello lagoon (Central Italy)

MESSINI, ANNA;PARISI, GIULIANA;FRATINI, GLENDA
2011

Abstract

Shellfish are perishable products due to their biochemical composition. Under refrigerated storage conditions, the shelf life of these products is limited by microbiological and enzymatic spoilage. Aim of the work was the evaluation of the influence of culture site on quality and safety of oysters and mussels, during the post-harvest refrigerated storage. In this study, 145 oysters and 90 mussels, sampled in Orbetello Lagoon (Italy), at March and at September respectively, were monitored during the storage at 4°C. The shellfish were analysed at 1, 3, 7 and 10 days from the harvesting by counting of aerobic psychrotrophic bacterial, pseudomonads, coliform bacteria and Escherichia coli. In order to deepen the knowledge about the role of pseudomonads, the significant number of strains from oysters and mussels were isolated. Total DNA was extracted from the isolates and 16S rDNA was PCR-amplified using FD1 and RD1 primers. Amplicons were subjected to ARDRA analysis using the restriction endonuclease CfoI. Representative strains of ARDRA groups were identified by 16S rDNA sequencing. In oysters, the aerobic psychrotrophic bacterial counts was 4.5 and 6.0 log CFUg–1 on the 1st and the 10th day, respectively; the pseudomonads values was 3.2 and 5.3 log CFUg–1 on the 1st and the 10th day respectively. In mussels, the aerobic psychrotrophic bacterial count was 5.8 and 6.2 log CFUg–1 on the 1st and the 10th day, respectively; the pseudomonads value was 4.2 and 6.2 log CFUg–1 on the 1st and the 10th day respectively. The coliform number was always below the limit permitted by the Italian law and Escherichia coli was always undetected. 16S rDNA sequencing indicated that the analysed isolates (pseudomonads), from oysters and safety mussels, belong to Pseudomonas spp.. Oysters and mussels maintained appreciable quality and safety characteristics during ten days of refrigerated storage.
2011
ASPA XIX Congress Book of Abstracts
XIX Congress of the Animal Science and Production Association A.S.P.A.
Cremona
A.MESSINI; G.PARISI; G. FRATINI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/474456
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