The effects of inhibitors of gamma-aminobutyric acid (GABA) metabolism or uptake on GABA output from the cerebral cortex was studied by means of a collecting cup placed on the exposed cortex of rats anaesthetized with urethane. GABA was identified and quantified by a mass-fragmentographic method. Ethanolamine-O-sulphate (10(-2) M) applied directly on the cerebral cortex caused a long-lasting twofold increase in GABA output, whereas DL-2,4-diaminobutyric acid (5 X 10(-3) M) caused a sevenfold increase and beta-alanine was active. The results indicate that glial uptake has little effect on GABA inactivation in the cerebral cortex. The inhibition of neuronal uptake seems a more effective tool to increase GABA concentration in the synaptic cleft, and consequently also in GABA output, than the inhibition of GABA metabolism.

In vitro changes in gamma-aminobutyric acid output from the cerebral cortex induced by inhibitors of gamma-aminobutyric acid uptake and metabolism / F. Moroni;A. Mulas;G. Moneti;G. Pepeu. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - STAMPA. - 39:(1982), pp. 582-584.

In vitro changes in gamma-aminobutyric acid output from the cerebral cortex induced by inhibitors of gamma-aminobutyric acid uptake and metabolism.

MORONI, FLAVIO;MONETI, GLORIANO;
1982

Abstract

The effects of inhibitors of gamma-aminobutyric acid (GABA) metabolism or uptake on GABA output from the cerebral cortex was studied by means of a collecting cup placed on the exposed cortex of rats anaesthetized with urethane. GABA was identified and quantified by a mass-fragmentographic method. Ethanolamine-O-sulphate (10(-2) M) applied directly on the cerebral cortex caused a long-lasting twofold increase in GABA output, whereas DL-2,4-diaminobutyric acid (5 X 10(-3) M) caused a sevenfold increase and beta-alanine was active. The results indicate that glial uptake has little effect on GABA inactivation in the cerebral cortex. The inhibition of neuronal uptake seems a more effective tool to increase GABA concentration in the synaptic cleft, and consequently also in GABA output, than the inhibition of GABA metabolism.
1982
39
582
584
F. Moroni;A. Mulas;G. Moneti;G. Pepeu
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/643157
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