The presence of amyloid-β (Aβ) fibrils is characteristic of Alzheimer's disease (AD), and the aggregation of these amyloidogenic proteins is a nucleation-dependent process. In this report, label-free methods based on surface plasmon resonance (SPR) and thickness shear mode acoustic wave sensors (TSM-AWS) were used to detect monomer elongation in real-time. The modulation of Aβ aggregation using a well-described flavonoid, clioquinol (CQ) was also observed. Established methods like fluorescence and electrochemistry were also employed to confirm the interaction of CQ with Aβ. Good correlation between the designed label-free methods creates a promising platform for the screening of novel amyloid inhibitors. © 2012 The Royal Society of Chemistry.

Label-free methods for probing the interaction of Clioquinol with Amyloid-β / Xinran Cheng; Vinci Wing Sze Hung; Simona Scarano; Marco Mascini; Maria Minunni; Kagan Kerman. - In: ANALYTICAL METHODS. - ISSN 1759-9660. - STAMPA. - 4:(2012), pp. 2228-2232. [10.1039/C2AY25123J]

Label-free methods for probing the interaction of Clioquinol with Amyloid-β

SCARANO, SIMONA;MINUNNI, MARIA;
2012

Abstract

The presence of amyloid-β (Aβ) fibrils is characteristic of Alzheimer's disease (AD), and the aggregation of these amyloidogenic proteins is a nucleation-dependent process. In this report, label-free methods based on surface plasmon resonance (SPR) and thickness shear mode acoustic wave sensors (TSM-AWS) were used to detect monomer elongation in real-time. The modulation of Aβ aggregation using a well-described flavonoid, clioquinol (CQ) was also observed. Established methods like fluorescence and electrochemistry were also employed to confirm the interaction of CQ with Aβ. Good correlation between the designed label-free methods creates a promising platform for the screening of novel amyloid inhibitors. © 2012 The Royal Society of Chemistry.
2012
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2228
2232
Xinran Cheng; Vinci Wing Sze Hung; Simona Scarano; Marco Mascini; Maria Minunni; Kagan Kerman
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/645151
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