In a previous paper, Mugnai et al. (2008) found that microgravity induced bursts of oxygen from maize (Zea mays L.) root apices detected by selective microelectrodes. We hypothesized that these bursts should be linked to change in root respiration; therefore we performed a series of experiments during the previous 43rd ESA PFC to monitor root respiration from excised roots by an oxymeter during the different gravity conditions of each parabola. Also, we used a metabolic inhibitor (D’orenon, Schlicht et al. 2008) able to depolarize F-actin filaments. As oxygen content inside the oxymeter chambers gradually decreased during the flight, results from the parabolas were divided into sub-groups related to the oxygen concentration in the chamber. Control apices generally showed an oxygen peak just after the beginning of g at low oxygen concentrations. The average (and sudden) oxygen production during these peaks was around 421 nM. The oxygen peak was followed by a temporary increment of respiration during g in control apices. On the contrary, apices treated with D’orenon did not show any changes neither in any peak appearance nor in root respiration, confirming the important role of F-actin filaments in the gravisensing process.

Root respiration under microgravity / C.Pandolfi; E.Masi; E.Azzarello; S.Mugnai; S.Mancuso. - STAMPA. - (2011), pp. 1-2. (Intervento presentato al convegno Biennial International Symposium of ELGRA (European Low Gravity Research Association) tenutosi a Antwerp (Belgio) nel 6-9 Sept 2011).

Root respiration under microgravity.

PANDOLFI, CAMILLA;MASI, ELISA;AZZARELLO, ELISA;MUGNAI, SERGIO;MANCUSO, STEFANO
2011

Abstract

In a previous paper, Mugnai et al. (2008) found that microgravity induced bursts of oxygen from maize (Zea mays L.) root apices detected by selective microelectrodes. We hypothesized that these bursts should be linked to change in root respiration; therefore we performed a series of experiments during the previous 43rd ESA PFC to monitor root respiration from excised roots by an oxymeter during the different gravity conditions of each parabola. Also, we used a metabolic inhibitor (D’orenon, Schlicht et al. 2008) able to depolarize F-actin filaments. As oxygen content inside the oxymeter chambers gradually decreased during the flight, results from the parabolas were divided into sub-groups related to the oxygen concentration in the chamber. Control apices generally showed an oxygen peak just after the beginning of g at low oxygen concentrations. The average (and sudden) oxygen production during these peaks was around 421 nM. The oxygen peak was followed by a temporary increment of respiration during g in control apices. On the contrary, apices treated with D’orenon did not show any changes neither in any peak appearance nor in root respiration, confirming the important role of F-actin filaments in the gravisensing process.
2011
ELGRA BIENNIAL SYMPOSIUM AND GENERAL ASSEMBLY 2011, Antwerp, Belgium Update 2 September 2011
Biennial International Symposium of ELGRA (European Low Gravity Research Association)
Antwerp (Belgio)
C.Pandolfi; E.Masi; E.Azzarello; S.Mugnai; S.Mancuso
File in questo prodotto:
File Dimensione Formato  
Abstract_ELGRA11.pdf

Accesso chiuso

Tipologia: Altro
Licenza: Tutti i diritti riservati
Dimensione 22.06 kB
Formato Adobe PDF
22.06 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/648652
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact