The development and use of Genetically Modified Organisms (GMO) as alternatives to traditional cultures underwent, during the last years, to a constant increase. However, the extensive introduction of GMOs into the environment has raised several problems due to their potential dangerous impact on the natural habitats, especially with regard to the probability of the transfer of the new genetic traits to the indigenous microorganisms, and the possibility that the new traits show a deleterious effect on the environment (Sharples, 1991). Recently, many experimental observations have indicated indicated that extracellular DNA, released in soil environment, can persist and maintain its transforming ability for long periods of time (Stotzky et al., 1996; Gallori et al., 1998). Aim of this work was to estimate the actual persistence in agricultural soils of transgenic plantDNA sequences, and their possible spreading to natural bacterial populations. For this purpose, we monitored, by PCR/Southern Blotting techniques, the presence of specific DNA markers in soil samples from transgenic cultures, evaluating the integrity of DNA molecules in time. Moreover, the genetic transformation of Bacillus subtilis competent cells with transgenic plant DNA, extracted directly from plants and/&or from soil samples is essayed.

Persistence of transgenic plant DNA in italian agricultural soils / M.FRANCHI;P. BOGANI; M. NARDI; E.PILLI; E. GALLORI. - STAMPA. - (2002), pp. 18-18. (Intervento presentato al convegno Symposium IGMO “The impact of GMOs: soil microbyology and nutrient Dynamics” tenutosi a Vienna, Austria nel November 4-6).

Persistence of transgenic plant DNA in italian agricultural soils.

BOGANI, PATRIZIA;E. PILLI;
2002

Abstract

The development and use of Genetically Modified Organisms (GMO) as alternatives to traditional cultures underwent, during the last years, to a constant increase. However, the extensive introduction of GMOs into the environment has raised several problems due to their potential dangerous impact on the natural habitats, especially with regard to the probability of the transfer of the new genetic traits to the indigenous microorganisms, and the possibility that the new traits show a deleterious effect on the environment (Sharples, 1991). Recently, many experimental observations have indicated indicated that extracellular DNA, released in soil environment, can persist and maintain its transforming ability for long periods of time (Stotzky et al., 1996; Gallori et al., 1998). Aim of this work was to estimate the actual persistence in agricultural soils of transgenic plantDNA sequences, and their possible spreading to natural bacterial populations. For this purpose, we monitored, by PCR/Southern Blotting techniques, the presence of specific DNA markers in soil samples from transgenic cultures, evaluating the integrity of DNA molecules in time. Moreover, the genetic transformation of Bacillus subtilis competent cells with transgenic plant DNA, extracted directly from plants and/&or from soil samples is essayed.
2002
Proc. Symp. IGMO “The impact of GMOs: soil microbyology and nutrient Dynamics”
Symposium IGMO “The impact of GMOs: soil microbyology and nutrient Dynamics”
Vienna, Austria
M.FRANCHI;P. BOGANI; M. NARDI; E.PILLI; E. GALLORI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/675338
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