Carbon-13 direct-detection NMR methods have proved to be very useful for the characterization of intrinsically disordered proteins (IDPs). Here we present a suite of experiments in which amino-acid-selective editing blocks are encoded in CACON- and CANCO-type sequences to give C-13-detected spectra containing correlations arising from a particular type or group of amino acid(s). These two general types of experiments provide the complementary intra- and inter-residue correlations necessary for sequence-specific assignment of backbone resonance frequencies. We demonstrate the capabilities of these experiments on two IDPs: fully reduced Cox17 and WIPC. The proposed approach constitutes an independent strategy to simplify crowded spectra as well as to perform sequence-specific assignment, thereby demonstrating its potential to study IDPs.

Exclusively Heteronuclear 13C-Detected Amino-Acid-Selective NMR Experiments for the Study of Intrinsically Disordered Proteins (IDPs) / W. Bermel; I. Bertini; J. Chill; I.C. Felli; N. Haba; M.V. Kumar; R. Pierattelli. - In: CHEMBIOCHEM. - ISSN 1439-4227. - STAMPA. - 13:(2012), pp. 2425-2432. [10.1002/cbic.201200447]

Exclusively Heteronuclear 13C-Detected Amino-Acid-Selective NMR Experiments for the Study of Intrinsically Disordered Proteins (IDPs)

BERTINI, IVANO;FELLI, ISABELLA CATERINA;MACHOHALLY VENKATESHAIAH, VASANTHA KUMAR;PIERATTELLI, ROBERTA
2012

Abstract

Carbon-13 direct-detection NMR methods have proved to be very useful for the characterization of intrinsically disordered proteins (IDPs). Here we present a suite of experiments in which amino-acid-selective editing blocks are encoded in CACON- and CANCO-type sequences to give C-13-detected spectra containing correlations arising from a particular type or group of amino acid(s). These two general types of experiments provide the complementary intra- and inter-residue correlations necessary for sequence-specific assignment of backbone resonance frequencies. We demonstrate the capabilities of these experiments on two IDPs: fully reduced Cox17 and WIPC. The proposed approach constitutes an independent strategy to simplify crowded spectra as well as to perform sequence-specific assignment, thereby demonstrating its potential to study IDPs.
2012
13
2425
2432
W. Bermel; I. Bertini; J. Chill; I.C. Felli; N. Haba; M.V. Kumar; R. Pierattelli
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/771285
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