Effect of low level laser irradiation on bone marrow mesenchymal stromal cells for bone tissue regeneration.

Reconstruction and rehabilitation of acquired defects or loss of the oral cavity is an important dental service. Severe deformities of the bone due to trauma, atrophy or periodontitis injuries may cause alveolar bone and soft tissue reduction and interfere with implant-prostetic rehabilitations. Mesenchymal stromal cells (MSCs) have been shown to be a promising source of adult stem cells for cell transplantation and bone repair/regeneration.The use of these cells in cell-therapy requires their expansion in vitro in order to obtain an adequate amount of cells to be implanted in the injured tissue . Therefore, improvements of their proliferative potential during in vitro culture can significantly shorten cell preparation time and avoid contamination, thus contributing to the further development of cell-based tissue regeneration. Low-Level Laser Irradiation (LLLI) may represent a promising approach to stimulate cell proliferation. In the present study we investigated the effects of 635 nm diode laser on mouse MSC proliferation and the underlying cellular and molecular mechanisms.