Abstract—Renal endothelin-1 participates in sodium and water handling, and its urinary excretion is increased in sodium-retentive states. We compared the cortical and medullary renal expression of prepro-endothelin-1, endothelinconverting enzyme-1, and endothelin type A and type B receptors in patients who underwent nephrectomy after normal (108 mmol/d NaCl; n6) or low (20 mmol/d NaCl; n6) sodium diet and investigated whether sodium exerts a direct role on endothelin receptor binding in vitro. With normal sodium diet prepro-endothelin-1 mRNA was 3-fold higher in renal medulla than in cortex (P0.01), whereas endothelin-converting enzyme-1 mRNA was equally distributed. Endothelin-1 receptor density was 2-fold higher in renal medulla than in cortex (P0.05). Type B was the main receptor subtype in both regions. In the renal cortex, low sodium diet caused a 194% increase in prepro-endothelin-1 mRNA (P0.05), whereas endothelin-converting enzyme-1 type B and type A receptors remained unchanged. In contrast, in the renal medulla the increase in prepro-endothelin-1 mRNA (30%, P0.05) was associated with a selective increase in type B receptor for both mRNA expression (37%, P0.05) and binding density (55%, P0.05). Increasing in vitro sodium concentrations between 154 and 308 mmol/L significantly enhanced type B receptor density (P0.05) and affinity (P0.05). In conclusion, during low sodium diet, renal prepro-endothelin-1 synthesis increases mainly in the renal cortex (where no changes in receptors occur), whereas type B receptor is selectively enhanced in the renal medulla. The range of sodium concentrations that are physiologically present in vivo in the renal medulla selectively modulate type B receptor density and affinity.

ETB Receptor in Renal Medulla Is Enhanced by Local Sodium During Low Salt Intake / S Vanni; G Polidori; I Cecioni; S Serni; M Carini; PA Modesti. - In: HYPERTENSION. - ISSN 0194-911X. - STAMPA. - 40:(2002), pp. 179-185. [10.1161/01.HYP.0000026809.68674.F9]

ETB Receptor in Renal Medulla Is Enhanced by Local Sodium During Low Salt Intake

VANNI, SIMONE;CECIONI, ILARIA;SERNI, SERGIO;CARINI, MARCO;MODESTI, PIETRO AMEDEO
2002

Abstract

Abstract—Renal endothelin-1 participates in sodium and water handling, and its urinary excretion is increased in sodium-retentive states. We compared the cortical and medullary renal expression of prepro-endothelin-1, endothelinconverting enzyme-1, and endothelin type A and type B receptors in patients who underwent nephrectomy after normal (108 mmol/d NaCl; n6) or low (20 mmol/d NaCl; n6) sodium diet and investigated whether sodium exerts a direct role on endothelin receptor binding in vitro. With normal sodium diet prepro-endothelin-1 mRNA was 3-fold higher in renal medulla than in cortex (P0.01), whereas endothelin-converting enzyme-1 mRNA was equally distributed. Endothelin-1 receptor density was 2-fold higher in renal medulla than in cortex (P0.05). Type B was the main receptor subtype in both regions. In the renal cortex, low sodium diet caused a 194% increase in prepro-endothelin-1 mRNA (P0.05), whereas endothelin-converting enzyme-1 type B and type A receptors remained unchanged. In contrast, in the renal medulla the increase in prepro-endothelin-1 mRNA (30%, P0.05) was associated with a selective increase in type B receptor for both mRNA expression (37%, P0.05) and binding density (55%, P0.05). Increasing in vitro sodium concentrations between 154 and 308 mmol/L significantly enhanced type B receptor density (P0.05) and affinity (P0.05). In conclusion, during low sodium diet, renal prepro-endothelin-1 synthesis increases mainly in the renal cortex (where no changes in receptors occur), whereas type B receptor is selectively enhanced in the renal medulla. The range of sodium concentrations that are physiologically present in vivo in the renal medulla selectively modulate type B receptor density and affinity.
2002
40
179
185
S Vanni; G Polidori; I Cecioni; S Serni; M Carini; PA Modesti
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/781166
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