Fibers and prebiotics represent a useful dietary approach for modulating the human gutmicrobiome. Therefore, aim of the present study was to investigate the impact of four flours (wholegrain rye, wholegrain wheat, chickpeas and lentils 50:50, and barleymilled grains), characterized by a naturally high content in dietary fibers, on the intestinal microbiota composition and metabolomic output. A validated three-stage continuous fermentative system simulating the human colon was used to resemble the complexity and diversity of the intestinal microbiota. Fluorescence in situ hybridization was used to evaluate the impact of the flours on the composition of the microbiota, while small-molecule metabolome was assessed by NMR analysis followed bymultivariate pattern recognition techniques. HT29 cell-growth curve assaywas used to evaluate the modulatory properties of the bacterial metabolites on the growth of intestinal epithelial cells. All the four flours showed positive modulations of the microbiota composition and metabolic activity. Furthermore, none of the flours influenced the growth-modulatory potential of the metabolites toward HT29 cells. Our findings support the utilization of the tested ingredients in the development of a variety of potentially prebiotic food products aimed at improving gastrointestinal health.

In Vitro fermentation of potentialprebiotic flours from natural sources: impact of the human colonic microbiota and metabolome / Maccaferri S.; Klinder A.; Cacciatore S.; Chitarrari R.; Honda H.; Luchinat C.; Bertini I.; Carnevali P.; Gibson G. F.; Brigidi P.; Costabile A.. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4125. - STAMPA. - 56:(2012), pp. 1342-1352. [10.1002/mnfr.201200046]

In Vitro fermentation of potentialprebiotic flours from natural sources: impact of the human colonic microbiota and metabolome

CACCIATORE, STEFANO;LUCHINAT, CLAUDIO;BERTINI, IVANO;
2012

Abstract

Fibers and prebiotics represent a useful dietary approach for modulating the human gutmicrobiome. Therefore, aim of the present study was to investigate the impact of four flours (wholegrain rye, wholegrain wheat, chickpeas and lentils 50:50, and barleymilled grains), characterized by a naturally high content in dietary fibers, on the intestinal microbiota composition and metabolomic output. A validated three-stage continuous fermentative system simulating the human colon was used to resemble the complexity and diversity of the intestinal microbiota. Fluorescence in situ hybridization was used to evaluate the impact of the flours on the composition of the microbiota, while small-molecule metabolome was assessed by NMR analysis followed bymultivariate pattern recognition techniques. HT29 cell-growth curve assaywas used to evaluate the modulatory properties of the bacterial metabolites on the growth of intestinal epithelial cells. All the four flours showed positive modulations of the microbiota composition and metabolic activity. Furthermore, none of the flours influenced the growth-modulatory potential of the metabolites toward HT29 cells. Our findings support the utilization of the tested ingredients in the development of a variety of potentially prebiotic food products aimed at improving gastrointestinal health.
2012
56
1342
1352
Maccaferri S.; Klinder A.; Cacciatore S.; Chitarrari R.; Honda H.; Luchinat C.; Bertini I.; Carnevali P.; Gibson G. F.; Brigidi P.; Costabile A.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/790537
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 55
  • ???jsp.display-item.citation.isi??? 47
social impact