A culture-independent PCR-based method was developed for the detection of L. thermotolerans during wine fermentations. This method, which utilizes species-specific PCR primer pairs that anneal to intron 2 of the mitochondrial COX1 gene, is rapid and reliable, and detects L. thermotolerans in wine at 104 cells/ml and with a S. cerevisiae/L. termotholerans ratio of 1,000/1
A culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine / Giacomo, Zara; Maurizio, Ciani; Domizio, Paola; Severino, Zara; Marilena, Budroni; Adriana, Carboni; Ilaria, Mannazzu. - In: ANNALS OF MICROBIOLOGY. - ISSN 1590-4261. - STAMPA. - 64:(2014), pp. 403-406. [10.1007/s13213-013-0647-4]
A culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine
DOMIZIO, PAOLA;
2014
Abstract
A culture-independent PCR-based method was developed for the detection of L. thermotolerans during wine fermentations. This method, which utilizes species-specific PCR primer pairs that anneal to intron 2 of the mitochondrial COX1 gene, is rapid and reliable, and detects L. thermotolerans in wine at 104 cells/ml and with a S. cerevisiae/L. termotholerans ratio of 1,000/1
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