The possible use of sterile triploid red tilapia is an interesting option for culture due to their proliferating breeding activities. The aim of the present study was to investigate and optimize the time of heat-shock treatment to prevent second polar body extrusion from the newly fertilized eggs of red tilapia, to produce a maximum number of triploid individuals. Heat-shock treatment was applied at a temperature of 41°C for a total duration of 3.5 minutes after 2, 3, 4, 4.5, 5 and 6 minutes of fertilization. The best survival rate (67.0%) and triploid percentage (89.7%) was observed for the treatment at 4 minutes after fertilization. It is thus successfully demonstrated that 4 minutes after fertilization was the most suitable timing of heat-shock treatment for second polar body retention in newly fertilized eggs of red tilapia.
Triploidy induction by heat-shock treatment in red tilapia / Padmaja Jayaprasad Pradeep; Thekkeparambil Chandrabose Srijaya; Aneesh Bahuleyan; Renjith Kumar; Alessio Papini; Anil Kumar Chatterji. - In: CARYOLOGIA. - ISSN 0008-7114. - STAMPA. - 65(2):(2012), pp. 152-156. [10.1080/00087114.2012.711678]
Triploidy induction by heat-shock treatment in red tilapia
PAPINI, ALESSIO;
2012
Abstract
The possible use of sterile triploid red tilapia is an interesting option for culture due to their proliferating breeding activities. The aim of the present study was to investigate and optimize the time of heat-shock treatment to prevent second polar body extrusion from the newly fertilized eggs of red tilapia, to produce a maximum number of triploid individuals. Heat-shock treatment was applied at a temperature of 41°C for a total duration of 3.5 minutes after 2, 3, 4, 4.5, 5 and 6 minutes of fertilization. The best survival rate (67.0%) and triploid percentage (89.7%) was observed for the treatment at 4 minutes after fertilization. It is thus successfully demonstrated that 4 minutes after fertilization was the most suitable timing of heat-shock treatment for second polar body retention in newly fertilized eggs of red tilapia.File | Dimensione | Formato | |
---|---|---|---|
77Prad_triploidyCaryo65_2_152_156.pdf
Accesso chiuso
Tipologia:
Versione finale referata (Postprint, Accepted manuscript)
Licenza:
Tutti i diritti riservati
Dimensione
109.13 kB
Formato
Adobe PDF
|
109.13 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.