Purification protocols for natural and recombinant allergens were established and quality assessment of the purified batches performed. Depending on either natural or recombinant origin different quality criteria were defined and met for inclusion into the allergen library. Structural integrity of the purified proteins was verified and IgE binding activity was assessed. This panel of well characterized allergens will be used to prove the concept of component resolved diagnosis and presents the necessary tools for setting up novel diagnostics. Using a significant number of allergic patients sera across Europe it will contribute to identify relevant sensitisation patterns and to improve dietary recommendations for the food allergic pa- tient in the future.
The europrevall allergen library: a collection of high quality purified natural and recombinant allergens for in vitro diagnosis / Hoffmann-Sommergruber, K ; Sancho, A ; Holzhauser, T; Skov, P; Alessandri, S; Wal, J ; Rigby, N; Marsh, J ; Bernard, H ; Gaier, S ; Lauer, I ; Ma, Y ; Oberhuber, C ; Barkholt, V; Griesmeier, U ; Bublin, M ; Radauer, C ; Scheurer, S Reese, G ; Zuidmeer, L; Akkerdaas, J; Neubauer, A ; Fernandez-Rivas, M ; Ballmer-Weber, B ; Vieths, S; van Ree, R ; Shewry, P ; Mills, C. - In: ALLERGY. - ISSN 0105-4538. - STAMPA. - 62 s83:(2007), pp. 343-343. [10.1111/j.1398-9995.2007.01407.964]
The europrevall allergen library: a collection of high quality purified natural and recombinant allergens for in vitro diagnosis
ALESSANDRI, STEFANO;
2007
Abstract
Purification protocols for natural and recombinant allergens were established and quality assessment of the purified batches performed. Depending on either natural or recombinant origin different quality criteria were defined and met for inclusion into the allergen library. Structural integrity of the purified proteins was verified and IgE binding activity was assessed. This panel of well characterized allergens will be used to prove the concept of component resolved diagnosis and presents the necessary tools for setting up novel diagnostics. Using a significant number of allergic patients sera across Europe it will contribute to identify relevant sensitisation patterns and to improve dietary recommendations for the food allergic pa- tient in the future.File | Dimensione | Formato | |
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