Background: The microvascular involvement of SSc is characterized by endothelial damage and impairment of endothelium-dependent vasodilation. Nitric oxide (NO) and Angiotensin Convertase Enzyme (ACE) are released by endothelial cells and contribute to vascular tone control. Objectives: To investigate the relationship between endothelium-dependent vasodilation and its correlation with nitric oxide and components Angiotensin system. Methods: Endothelium-dependent dilation of the brachial artery was measured as the percentage of change from baseline (FMD%) in 14 SSc patients (mean of age 60.25 ± 9.46 years, duration of disease was 7.87 ± 7.64 years, 3 subset diffuse and 11 limited subset) and 11 age-and sex-matched healthy controls by high-resolution ultrasound imaging. In the same patients, plasma nitrate levels, the most important metabolite of nitric oxide was evaluated by ELISA kit. Serum levels of other peptides contribuiting to vascular tone control, were also measured: Angiotensin I (AI), Angiotensin II (AII), Angiotensin 1-7 (A1-7) and ACE. Results: Brachial artery FMD% differ between SSc patients and controls (mean 3,66 ± 3,47% versus 8,4 ± 3,9% respectively, p<0,001) and correlated significantly with NO levels (p<0,001). NO levels are significantly lower than controls (24,82±11,56 vs 34,03±9,39 mM p<0,01). FMD All components of the angiotensin system are significantly reduced in SSc (ACE 408,4± 126,2 vs 593,9±58,96 ng/ml p<0,001; AI 25,86±11,11 vs 39,71±17,24 pg/ml p<0,001; AII 11,40±3,55 vs 19,79±7,36 pg/ml p<0,01; A1-7 9,08±2,02 vs 30,36±22,4 pg/ml p<0,001). Conclusion: The ability of the brachial arteries to dilate in response to hyperemia is reduced in SSc and seems correlated to the reduction of circulating NO. Hyperemia induced low levels of FMD% and the low levels of NO and angiotensin system components suggest a widespred endothelial dysfunction in SSc patients.

ENDOTHELIAL DYSFUNCTION CORRELATE WITH REDUCED NITRIC OXIDE PRODUCTION IN SYSTEMIC SCLEROSIS (SSC) / F. Bartoli; M. Bacci; S. Chiarpellini; S. Guiducci; M. Cinelli; A. Del Rosso; G. Fiori; S. Generini; A. Pignone; S. Castellani; M. Matucci-Cerinic. - In: ANNALS OF THE RHEUMATIC DISEASES. - ISSN 0003-4967. - STAMPA. - 64 (Suppl.III):(2005), pp. 282-282.

ENDOTHELIAL DYSFUNCTION CORRELATE WITH REDUCED NITRIC OXIDE PRODUCTION IN SYSTEMIC SCLEROSIS (SSC)

GUIDUCCI, SERENA;MOGGI PIGNONE, ALBERTO;S. Castellani;
2005

Abstract

Background: The microvascular involvement of SSc is characterized by endothelial damage and impairment of endothelium-dependent vasodilation. Nitric oxide (NO) and Angiotensin Convertase Enzyme (ACE) are released by endothelial cells and contribute to vascular tone control. Objectives: To investigate the relationship between endothelium-dependent vasodilation and its correlation with nitric oxide and components Angiotensin system. Methods: Endothelium-dependent dilation of the brachial artery was measured as the percentage of change from baseline (FMD%) in 14 SSc patients (mean of age 60.25 ± 9.46 years, duration of disease was 7.87 ± 7.64 years, 3 subset diffuse and 11 limited subset) and 11 age-and sex-matched healthy controls by high-resolution ultrasound imaging. In the same patients, plasma nitrate levels, the most important metabolite of nitric oxide was evaluated by ELISA kit. Serum levels of other peptides contribuiting to vascular tone control, were also measured: Angiotensin I (AI), Angiotensin II (AII), Angiotensin 1-7 (A1-7) and ACE. Results: Brachial artery FMD% differ between SSc patients and controls (mean 3,66 ± 3,47% versus 8,4 ± 3,9% respectively, p<0,001) and correlated significantly with NO levels (p<0,001). NO levels are significantly lower than controls (24,82±11,56 vs 34,03±9,39 mM p<0,01). FMD All components of the angiotensin system are significantly reduced in SSc (ACE 408,4± 126,2 vs 593,9±58,96 ng/ml p<0,001; AI 25,86±11,11 vs 39,71±17,24 pg/ml p<0,001; AII 11,40±3,55 vs 19,79±7,36 pg/ml p<0,01; A1-7 9,08±2,02 vs 30,36±22,4 pg/ml p<0,001). Conclusion: The ability of the brachial arteries to dilate in response to hyperemia is reduced in SSc and seems correlated to the reduction of circulating NO. Hyperemia induced low levels of FMD% and the low levels of NO and angiotensin system components suggest a widespred endothelial dysfunction in SSc patients.
2005
F. Bartoli; M. Bacci; S. Chiarpellini; S. Guiducci; M. Cinelli; A. Del Rosso; G. Fiori; S. Generini; A. Pignone; S. Castellani; M. Matucci-Cerinic
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/823211
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