Formation of amyloid-beta (Abeta){1-42} amyloid fibrils, a characteristic feature of Alzheimer's disease (AD), was monitored in situ through atomic force microscopy (AFM). Well-structured amyloid fibrils slowly formed in solution within 24 hours for which high quality AFM pictures could be obtained. Remarkably, addition of either copper(II) or zinc(II) ions to the incubation medium, even at extremely low molar ratios, dramatically changed the Abeta {1-42} aggregation profile and prevented fibril formation. Aggregates of different morphology appeared in accordance with previous observations: small globular aggregates upon addition of zinc; ill-structured micro-aggregates in the case of copper. The implications of these AFM results are discussed in the context of current concepts for AD metallobiology.

Trace Copper(II) or Zinc(II) Ions Drastically Modify the Aggregation Behavior of Amyloid-beta(1-42): An AFM Study / Innocenti, M.; Salvietti, E.; Guidotti, M.; Casini, A.; Belleandi, S.; Foresti, Ml.; Gabbiani, C.; Pozzi, A.; Zatta, P.; Messori, L.. - In: JOURNAL OF ALZHEIMER'S DISEASE. - ISSN 1387-2877. - ELETTRONICO. - 19:(2010), pp. 1323-1329.

Trace Copper(II) or Zinc(II) Ions Drastically Modify the Aggregation Behavior of Amyloid-beta(1-42): An AFM Study

INNOCENTI, MASSIMO;SALVIETTI, EMANUELE;GUIDOTTI, MARTINA;CASINI, ANGELA;FORESTI, MARIA LUISA;GABBIANI, CHIARA;POZZI, PIER ANDREA;MESSORI, LUIGI;BELLANDI, SILVANO
2010

Abstract

Formation of amyloid-beta (Abeta){1-42} amyloid fibrils, a characteristic feature of Alzheimer's disease (AD), was monitored in situ through atomic force microscopy (AFM). Well-structured amyloid fibrils slowly formed in solution within 24 hours for which high quality AFM pictures could be obtained. Remarkably, addition of either copper(II) or zinc(II) ions to the incubation medium, even at extremely low molar ratios, dramatically changed the Abeta {1-42} aggregation profile and prevented fibril formation. Aggregates of different morphology appeared in accordance with previous observations: small globular aggregates upon addition of zinc; ill-structured micro-aggregates in the case of copper. The implications of these AFM results are discussed in the context of current concepts for AD metallobiology.
2010
19
1323
1329
Innocenti, M.; Salvietti, E.; Guidotti, M.; Casini, A.; Belleandi, S.; Foresti, Ml.; Gabbiani, C.; Pozzi, A.; Zatta, P.; Messori, L.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/975791
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