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Advanced structural and morphological characterization by NMR technologies of different biomolecules and biotechnological systems

This research work focuses on the advanced characterization of pharmaceutical systems which are used as delivery platforms for nucleic acids and proteins, using advanced Nuclear Magnetic Resonance (NMR) techniques. The delivery systems studied in this doctoral work are liposomes, Lipid Nanoparticles (LNPs) and Red Blood Cells (RBC). There is indeed an urgent need to enhance understanding of structural attributes that can be linked to functional variations, thereby guiding rational formul...

19F in-cell NMR to investigate protein-ligand interactions in living human cells.

In this PhD project, in-cell 19F NMR spectroscopy approaches were developed and applied to investigate target–ligand interactions directly in living human cells. Two complementary strategies were pursued: a target-based approach and a ligand-based approach. The target-based approach focused on expressing fluorinated proteins in HEK293T cells by controlling the incorporation of fluorinated aromatic amino acids. The study showed that fluorine incorporation occurs stochastically, resulting in a...

Optimisation of municipal solid waste collection to enhance materials recovery

Municipal Solid Waste (MSW) management plays a critical role in advancing the goals of the circular economy and climate neutrality across the European Union. Within this framework, collection is not merely a logistical task, but a strategic lever for enhancing material recovery, reducing environmental impacts, and improving system-wide efficiency. Despite its central role, MSW collection is still frequently approached as an operational routine, with limited integration into planning and p...

Incorporation of natural and non-natural amino acids in human cells for advanced in-cell NMR labelling schemes

This thesis presents significant advancements in developing selective isotopic labelling strategies for in-cell nuclear magnetic resonance (NMR) spectroscopy within human cells, aiming to overcome limitations related to cost, sensitivity, specificity, applicability to complex biological systems and physiological models. We first established a cost-effective and innovative methodology for selective side-chain isotope labelling in mammalian cells. This method exploits the activity of human cel...

A Semanti Digital Twin for Occupant-Centric Workplace Management

Providing high-quality indoor workplaces that meet occupants’ needs and support their activities is essential for employee health, well-being, and productivity. Established Facility Management (FM) approaches—focused on monitoring environmental parameters and viewing occupants as passive recipient of averaged ideal conditions—have shown limited success in fully meeting these expectations. Additionally, conventional Post-Occupancy Evaluation (POE) surveys, which often lack the contextual data ...

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