Extracellular Vesicles (EVs) - cell secreted vesicles that carry rich molecular information of the parental cell and constitute an important mode of intercellular communication - are becoming a primary topic in translational medicine. EVs (that comprise exosomes and microvesicles/microparticles) have a size ranging from 40 nm to 1 mu m and share several physicochemical proprieties, including size, density, surface charge, and light interaction, with other nano-objects present in body fluids, such as single and aggregated proteins. This makes separation, titration, and characterization of EVs challenging and time-consuming. Here we present a cost-effective and fast colorimetric assay for probing by eye protein contaminants and determine the concentration of EV preparations, which exploits the synergy between colloidal gold nanoplasmonics, nanoparticleprotein corona, and nanoparticlemembrane interaction. The assay hits a limit of detection of protein contaminants of 5 ng/mu L and has a dynamic range of EV concentration ranging from 35 fM to 35 pM, which matches the typical range of EV concentration in body fluids. This work provides the first example of the exploitation of the nanoparticleprotein corona in analytical chemistry.

Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles / Maiolo, Daniele; Paolini, Lucia; Di Noto, Giuseppe; Zendrini, Andrea; Berti, Debora; Bergese, Paolo; Ricotta, Doris. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - STAMPA. - 87:(2015), pp. 4168-4176. [10.1021/ac504861d]

Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles

BERTI, DEBORA;
2015

Abstract

Extracellular Vesicles (EVs) - cell secreted vesicles that carry rich molecular information of the parental cell and constitute an important mode of intercellular communication - are becoming a primary topic in translational medicine. EVs (that comprise exosomes and microvesicles/microparticles) have a size ranging from 40 nm to 1 mu m and share several physicochemical proprieties, including size, density, surface charge, and light interaction, with other nano-objects present in body fluids, such as single and aggregated proteins. This makes separation, titration, and characterization of EVs challenging and time-consuming. Here we present a cost-effective and fast colorimetric assay for probing by eye protein contaminants and determine the concentration of EV preparations, which exploits the synergy between colloidal gold nanoplasmonics, nanoparticleprotein corona, and nanoparticlemembrane interaction. The assay hits a limit of detection of protein contaminants of 5 ng/mu L and has a dynamic range of EV concentration ranging from 35 fM to 35 pM, which matches the typical range of EV concentration in body fluids. This work provides the first example of the exploitation of the nanoparticleprotein corona in analytical chemistry.
2015
87
4168
4176
Goal 3: Good health and well-being for people
Maiolo, Daniele; Paolini, Lucia; Di Noto, Giuseppe; Zendrini, Andrea; Berti, Debora; Bergese, Paolo; Ricotta, Doris
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1006541
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