Fibroblasts are the most abundant cells in connective tissue and, with fibrillar extracellular matrix, form the structural scaffolding of organs. In solid tumors, interaction with cancer cells induces fibroblasts transdifferentiation into an activated form, which become a fundamental part of the tumor stroma. Within tumor microenvironment stromal and cancer cells engage a crosstalk that is mediated by soluble factors, cell–cell contacts and extracellular vesicles trafficking. Here we report that fibroblasts have the ability to transfer a remarkable amount of proteins and lipids to neighboring cells, in an ectosome-dependent fashion, identifying a novel and native property of these cells. Cancer-associated fibroblasts show an enhanced production and delivering of ectosomes to cancer cells compared to normal fibroblasts. As a consequence of this phenomenon, tumor cells increase their proliferation rate, indicating that ectosome-mediated trafficking could be a relevant mechanism mediating the trophic function of activated connective tissue on tumor cells.

Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth / Santi, A; Caselli, A; Ranaldi, F; Paoli, P; Mugnaioni, C; Michelucci, E; Cirri, P. - In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. - ISSN 0167-4889. - ELETTRONICO. - 1853:(2015), pp. 3211-3223. [10.1016/j.bbamcr.2015.09.013]

Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth

SANTI, ALICE
Investigation
;
CASELLI, ANNA
Investigation
;
RANALDI, FRANCESCO
Membro del Collaboration Group
;
PAOLI, PAOLO
Membro del Collaboration Group
;
MUGNAIONI, CAMILLA
Membro del Collaboration Group
;
MICHELUCCI, ELENA
Membro del Collaboration Group
;
CIRRI, PAOLO
Supervision
2015

Abstract

Fibroblasts are the most abundant cells in connective tissue and, with fibrillar extracellular matrix, form the structural scaffolding of organs. In solid tumors, interaction with cancer cells induces fibroblasts transdifferentiation into an activated form, which become a fundamental part of the tumor stroma. Within tumor microenvironment stromal and cancer cells engage a crosstalk that is mediated by soluble factors, cell–cell contacts and extracellular vesicles trafficking. Here we report that fibroblasts have the ability to transfer a remarkable amount of proteins and lipids to neighboring cells, in an ectosome-dependent fashion, identifying a novel and native property of these cells. Cancer-associated fibroblasts show an enhanced production and delivering of ectosomes to cancer cells compared to normal fibroblasts. As a consequence of this phenomenon, tumor cells increase their proliferation rate, indicating that ectosome-mediated trafficking could be a relevant mechanism mediating the trophic function of activated connective tissue on tumor cells.
1853
3211
3223
Santi, A; Caselli, A; Ranaldi, F; Paoli, P; Mugnaioni, C; Michelucci, E; Cirri, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1009088
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