DNA integrity is a critical part of the definition of genomic DNA (gDNA) quality and can influence downstream molecular applications. Pre-analytical variables as sample storage and DNA extraction methods can influence the quality and quantity of isolated DNA and affect molecular test performances. The aim of this paper is to investigate the role of blood sample storage and DNA extraction procedures on gDNA integrity and gDNA fragmentation impact on a molecular test.
Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience / Malentacchi, F; Ciniselli, Cm; Pazzagli, M; Verderio, P; Barraud, L; Hartmann, Cc; Pizzamiglio, S; Weisbuch, S; Wyrich, R; Gelmini, S. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 440:(2015), pp. 205-210. [10.1016/j.cca.2014.12.004]
Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience
MALENTACCHI, FRANCESCA;PAZZAGLI, MARIO;GELMINI, STEFANIA
2015
Abstract
DNA integrity is a critical part of the definition of genomic DNA (gDNA) quality and can influence downstream molecular applications. Pre-analytical variables as sample storage and DNA extraction methods can influence the quality and quantity of isolated DNA and affect molecular test performances. The aim of this paper is to investigate the role of blood sample storage and DNA extraction procedures on gDNA integrity and gDNA fragmentation impact on a molecular test.
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
