This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NEU) with protease activtiy and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere.

Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency / Baraniya, Divyashri; Puglisi, Edoardo; Ceccherini, Maria Teresa; Pietramellara, Giacomo; Giagnoni, Laura; Arenella, Mariarita; Nannipieri, Paolo; Renella, Giancarlo. - In: SOIL BIOLOGY & BIOCHEMISTRY. - ISSN 0038-0717. - STAMPA. - 96:(2016), pp. 176-179. [10.1016/j.soilbio.2016.02.001]

Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency

CECCHERINI, MARIA TERESA;PIETRAMELLARA, GIACOMO;GIAGNONI, LAURA;ARENELLA, MARIARITA;NANNIPIERI, PAOLO;
2016

Abstract

This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NEU) with protease activtiy and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere.
2016
96
176
179
Baraniya, Divyashri; Puglisi, Edoardo; Ceccherini, Maria Teresa; Pietramellara, Giacomo; Giagnoni, Laura; Arenella, Mariarita; Nannipieri, Paolo; Renella, Giancarlo
File in questo prodotto:
File Dimensione Formato  
Baraniya et al., 2016_SBB.pdf

Accesso chiuso

Descrizione: Articolo principale
Tipologia: Pdf editoriale (Version of record)
Licenza: DRM non definito
Dimensione 769.37 kB
Formato Adobe PDF
769.37 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1028042
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 35
social impact