Autophagy maintains intracellular homeostasis during placental development and embryogenesis. We evaluated if differences in the autophagy-inducing capacity of sera from women undergoing an in vitro fertilization (IVF) cycle predicted subsequent pregnancy outcome. In this retrospective study, sera collected from 94 women at the time of intrauterine embryo implantation were incubated with peripheral blood mononuclear cells (PBMCs) from healthy donors. The PBMCs were lysed and autophagy induction measured by determination of the p62 concentration. A reduced capacity for autophagy induction was associated with defective implantation while an elevated level of autophagy was associated with ectopic pregnancy.

Autophagy induction by sera from women undergoing an in vitro fertilization cycle varies with subsequent outcome / Sisti, Giovanni; Kanninen, Tomi T.; Di Tommaso, Mariarosaria; Witkin, Steven S.; Spandorfer, Steven D.. - In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY. - ISSN 0165-0378. - ELETTRONICO. - 117:(2016), pp. 1-3. [10.1016/j.jri.2016.06.003]

Autophagy induction by sera from women undergoing an in vitro fertilization cycle varies with subsequent outcome

SISTI, GIOVANNI;DI TOMMASO, MARIAROSARIA;
2016

Abstract

Autophagy maintains intracellular homeostasis during placental development and embryogenesis. We evaluated if differences in the autophagy-inducing capacity of sera from women undergoing an in vitro fertilization (IVF) cycle predicted subsequent pregnancy outcome. In this retrospective study, sera collected from 94 women at the time of intrauterine embryo implantation were incubated with peripheral blood mononuclear cells (PBMCs) from healthy donors. The PBMCs were lysed and autophagy induction measured by determination of the p62 concentration. A reduced capacity for autophagy induction was associated with defective implantation while an elevated level of autophagy was associated with ectopic pregnancy.
2016
117
1
3
Sisti, Giovanni; Kanninen, Tomi T.; Di Tommaso, Mariarosaria; Witkin, Steven S.; Spandorfer, Steven D.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1048802
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