The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu2+) or a non-essential metal (Cd2+),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece's proteomes were evaluated after chronic and acute exposure to Cu2+ and Cd2+ in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1mg/l of Cu2+ or 5mg/l of Cd2+ were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24h. 202 and 268 proteins were identified and quantified for studies 1 (Cu2+) and 2 (Cd2+), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism
Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals / Mota, Rita; Pereira, Sara B.; Meazzini, Marianna; Fernandes, Rui; Santos, Arlete; Evans, Caroline A.; De Philippis, Roberto; Wright, Phillip C.; Tamagnini, Paula. - In: DATA IN BRIEF. - ISSN 2352-3409. - STAMPA. - 4:(2015), pp. 152-158. [10.1016/j.dib.2015.04.015]
Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals
DE PHILIPPIS, ROBERTO;
2015
Abstract
The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu2+) or a non-essential metal (Cd2+),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece's proteomes were evaluated after chronic and acute exposure to Cu2+ and Cd2+ in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1mg/l of Cu2+ or 5mg/l of Cd2+ were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24h. 202 and 268 proteins were identified and quantified for studies 1 (Cu2+) and 2 (Cd2+), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolismFile | Dimensione | Formato | |
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