Sterile Insect Technique (SIT) is a powerful tool for in modern IPM strategies that has been applied to control several insect pests, especially fruit flies. Presently SIT cannot be employed to control the olive fly, the primary insect pest of olive fruits, mainly due to the high cost procedure and low quality of mass reared flies. Lack of symbiotic microorganisms in lab reared colonies might be the limiting factor in olive fly mass production. Since the use of preservatives and antimicrobials in artificial diets likely causes the olive fly to lose its endosymbiont, Candidatus Erwinia dacicola, the hypothesis that the role of the symbiont may be replaced in lab reared flies by supplying culturable bacteria was explored. The effects of bacteria on the olive fly physiology were evaluated feeding adults of a Bactrocera oleae lab strain with probiotic diets based on two bacteria that have been found associated with the fly: Pseudomonas putida and Acetobacter tropicalis. In order to highlight possible probiotic effects of the microorganisms on the olive fly fitness, live and heath-killed bacteria have been supplied at two different concentrations. Laboratory bioassays showed how live P. putida colonies positively affected longevity and fecundity, moreover the female survival was increased by the twofold concentration of the bacterium. On the contrary, probiotic diet containing heath-killed P. putida did not positively affect adult life span, indirectly proving that live bacteria are involved in the host metabolism and consequently they may play an important role within the host gut. In general, flies fed on live A. tropicalis showed a negative effect on olive fly survival compared to those fed on sugar, although the probiotic diet with the bacterium at the lower concentration enhanced egg production. Concerning fecundity, flies fed on full protein diet always laid the highest number of eggs, compared to sugar or to probiotic diets.

Effects of probiotic diets on the olive fly fitness / Sacchetti, P.; Becherini, L.; Belcari, A.. - STAMPA. - (2015), pp. 35-35. (Intervento presentato al convegno 7th IOBC/WPRS Integrated protection of olive crops tenutosi a Kalamata, Greece nel 11-14 May 2015).

Effects of probiotic diets on the olive fly fitness

SACCHETTI, PATRIZIA;BELCARI, ANTONIO
2015

Abstract

Sterile Insect Technique (SIT) is a powerful tool for in modern IPM strategies that has been applied to control several insect pests, especially fruit flies. Presently SIT cannot be employed to control the olive fly, the primary insect pest of olive fruits, mainly due to the high cost procedure and low quality of mass reared flies. Lack of symbiotic microorganisms in lab reared colonies might be the limiting factor in olive fly mass production. Since the use of preservatives and antimicrobials in artificial diets likely causes the olive fly to lose its endosymbiont, Candidatus Erwinia dacicola, the hypothesis that the role of the symbiont may be replaced in lab reared flies by supplying culturable bacteria was explored. The effects of bacteria on the olive fly physiology were evaluated feeding adults of a Bactrocera oleae lab strain with probiotic diets based on two bacteria that have been found associated with the fly: Pseudomonas putida and Acetobacter tropicalis. In order to highlight possible probiotic effects of the microorganisms on the olive fly fitness, live and heath-killed bacteria have been supplied at two different concentrations. Laboratory bioassays showed how live P. putida colonies positively affected longevity and fecundity, moreover the female survival was increased by the twofold concentration of the bacterium. On the contrary, probiotic diet containing heath-killed P. putida did not positively affect adult life span, indirectly proving that live bacteria are involved in the host metabolism and consequently they may play an important role within the host gut. In general, flies fed on live A. tropicalis showed a negative effect on olive fly survival compared to those fed on sugar, although the probiotic diet with the bacterium at the lower concentration enhanced egg production. Concerning fecundity, flies fed on full protein diet always laid the highest number of eggs, compared to sugar or to probiotic diets.
2015
Abstract book of the 7th IOBC/WPRS Integrated protection of olive crops, Kalamata, Greece, 11-14 May 2015
7th IOBC/WPRS Integrated protection of olive crops
Kalamata, Greece
Sacchetti, P.; Becherini, L.; Belcari, A.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1051087
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