The Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a model organisms of cold-adapted bacteria. The interest in the study of this psychrophilic bacterium stems from its capability either as non-conventional system for production of recombinant protein and as a rich source of bioactive compounds. To further explore the biotechnological ability of P. haloplanktis TAC125, we have developed a synthetic medium, containing D-gluconate and L-glutamate (GG), which allows the bacterium to grow even at subzero temperatures. P. haloplanktis TAC125 growing in GG medium at low temperature displays growth kinetic parameters which confirm its spectacular adaptation to cold environment and subzero lifestyle, paving the way to the definition of the underlying molecular strategies. Moreover, in this paper we report the setup of a finely regulated gene-expression system inducible by D-galactose to produce recombinant protein in GG synthetic medium at temperatures as low as -2,5°C. Thanks to the combination of the novel medium and the new expression system, we obtained for the first time the production of a recombinant protein at subzero temperature, thus providing an innovative strategy for the recombinant production of “difficult” proteins.

A novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Sannino, F.; Giuliani, M.; Salvatore, U.; Apuzzo, G. A.; de Pascale, D.; Fani, Renato; Fondi, Marco; Marino, G.; Tutino, M. L.; Parrilli, E.. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - STAMPA. - 101:(2017), pp. 725-734.

A novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125

FANI, RENATO;FONDI, MARCO;
2017

Abstract

The Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a model organisms of cold-adapted bacteria. The interest in the study of this psychrophilic bacterium stems from its capability either as non-conventional system for production of recombinant protein and as a rich source of bioactive compounds. To further explore the biotechnological ability of P. haloplanktis TAC125, we have developed a synthetic medium, containing D-gluconate and L-glutamate (GG), which allows the bacterium to grow even at subzero temperatures. P. haloplanktis TAC125 growing in GG medium at low temperature displays growth kinetic parameters which confirm its spectacular adaptation to cold environment and subzero lifestyle, paving the way to the definition of the underlying molecular strategies. Moreover, in this paper we report the setup of a finely regulated gene-expression system inducible by D-galactose to produce recombinant protein in GG synthetic medium at temperatures as low as -2,5°C. Thanks to the combination of the novel medium and the new expression system, we obtained for the first time the production of a recombinant protein at subzero temperature, thus providing an innovative strategy for the recombinant production of “difficult” proteins.
2017
101
725
734
Sannino, F.; Giuliani, M.; Salvatore, U.; Apuzzo, G. A.; de Pascale, D.; Fani, Renato; Fondi, Marco; Marino, G.; Tutino, M. L.; Parrilli, E.
File in questo prodotto:
File Dimensione Formato  
Sannino et al 2017 - Applied Microbiology and Biotechnology - A novel synthetic medium for.pdf

Accesso chiuso

Descrizione: Sannino et al 2017
Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 645.2 kB
Formato Adobe PDF
645.2 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1055162
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 21
social impact