Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for long-term storage and reusability. Recently, it was shown that an atomic-level description of proteins immobilized in a biosilica matrix can be attained by examining their magic-angle spinning (MAS) NMR spectra. However, even though MAS NMR is an excellent tool for determining structure, it is severely hampered by sensitivity. In this work we provide the proof of principle that NMR characterization of biosilica-entrapped enzymes could be assisted by high-field dynamic nuclear polarization (DNP).

Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy / Ravera, Enrico; Michaelis, Vladimir K.; Ong, Ta-Chung; Keeler, Eric G.; Martelli, Tommaso; Fragai, Marco; Griffin, Robert G.; Luchinat, Claudio. - In: CHEMPHYSCHEM. - ISSN 1439-4235. - STAMPA. - 16(2015), pp. 2751-2754. [10.1002/cphc.201500549]

Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy

RAVERA, ENRICO;MARTELLI, TOMMASO;FRAGAI, MARCO;LUCHINAT, CLAUDIO
2015

Abstract

Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for long-term storage and reusability. Recently, it was shown that an atomic-level description of proteins immobilized in a biosilica matrix can be attained by examining their magic-angle spinning (MAS) NMR spectra. However, even though MAS NMR is an excellent tool for determining structure, it is severely hampered by sensitivity. In this work we provide the proof of principle that NMR characterization of biosilica-entrapped enzymes could be assisted by high-field dynamic nuclear polarization (DNP).
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Ravera, Enrico; Michaelis, Vladimir K.; Ong, Ta-Chung; Keeler, Eric G.; Martelli, Tommaso; Fragai, Marco; Griffin, Robert G.; Luchinat, Claudio
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/1058395
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