The design and synthesis of the Ln3+ complexes of a DOTA containing (DOTA=1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) inhibitor of matrix metalloproteinases are reported. The tight binding of the sulfonamide scaffold to the catalytic domain of the investigated matrix metalloproteinase is not impaired by the presence of the Ln3+-DOTA moiety. The paramagnetic properties of the Ln3+ complex are exploited to obtain insights into the structural features of the ligand–protein interactions and to evaluate the influence of the linker length on the quality of the paramagnetic restraints.

Active-Site Targeting Paramagnetic Probe for Matrix Metalloproteinases / Baldoneschi, Veronica; Cerofolini, Linda; Dragoni, Elisa; Storai, Andrea; Luchinat, Claudio; Fragai, Marco; Richichi, Barbara; Nativi, Cristina. - In: CHEMPLUSCHEM. - ISSN 2192-6506. - ELETTRONICO. - 81:(2016), pp. 1333-1338. [10.1002/cplu.201600375]

Active-Site Targeting Paramagnetic Probe for Matrix Metalloproteinases

BALDONESCHI, VERONICA;CEROFOLINI, LINDA;DRAGONI, ELISA;LUCHINAT, CLAUDIO;FRAGAI, MARCO
;
RICHICHI, BARBARA
;
NATIVI, CRISTINA
2016

Abstract

The design and synthesis of the Ln3+ complexes of a DOTA containing (DOTA=1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) inhibitor of matrix metalloproteinases are reported. The tight binding of the sulfonamide scaffold to the catalytic domain of the investigated matrix metalloproteinase is not impaired by the presence of the Ln3+-DOTA moiety. The paramagnetic properties of the Ln3+ complex are exploited to obtain insights into the structural features of the ligand–protein interactions and to evaluate the influence of the linker length on the quality of the paramagnetic restraints.
2016
81
1333
1338
Baldoneschi, Veronica; Cerofolini, Linda; Dragoni, Elisa; Storai, Andrea; Luchinat, Claudio; Fragai, Marco; Richichi, Barbara; Nativi, Cristina...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1063834
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