On the reproducibility and repeatability of laser absorption spectroscopy measurements for δ2H and δ18O isotopic analysis

The aim of this study was to analyse the reproducibility of off-axis integrated cavity output spectroscopy (OA-ICOS)-derived delta H-2 and delta O-18 measurements on a set of 35 water samples by comparing the performance of four laser spectroscopes with the performance of a conventional mass spectrometer under typical laboratory conditions. All samples were analysed using three different schemes of standard/sample combinations and related data processing to assess the improvement of results compared with mass spectrometry. The repeatability of the four OA-ICOS instruments was further investigated by multiple analyses of a sample subset to evaluate the stability of delta H-2 and delta O-18 measurements. </br > Results demonstrated an overall agreement between OA-ICOS-based and mass spectrometry-based measurements for the entire dataset. However, a certain degree of variability existed in precision and accuracy between the four instruments. There was no evident bias or systematic deviations from the mass spectrometer values, but random errors, which were apparently not related to external factors, significantly affected the final results. Our investigation revealed that analytical precision ranged +/- from +/- 0.56 parts per thousand to +/- 1.80 parts per thousand for delta H-2 and from +/- 0.10 parts per thousand to +/- 0.27 parts per thousand for delta O-18 measurements, with a marked variability among the four instruments. The overall capability of laser instruments to reproduce stable results with repeated measurements of the same sample was acceptable, and there were general differences within the range of the analytical precision for each spectroscope. Hence, averaging the measurements of three identical samples led to a higher degree of accuracy and eliminated the potential for random deviations.