Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
Sensors - Proceedings of the 2nd National Conference on Sensors, 2014 / Voccia, D; Sosnowska, M.; Bettazzi, F.; Palchetti, I.; Kutner, W.. - STAMPA. - 319:(2015), pp. 59-64. (Intervento presentato al convegno 2nd National Conference on Sensors, 2014 tenutosi a ita nel 2014) [10.1007/978-3-319-09617-9_11].
Sensors - Proceedings of the 2nd National Conference on Sensors, 2014
BETTAZZI, FRANCESCA;PALCHETTI, ILARIA
;
2015
Abstract
Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
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